ORIGINAL PAPER
The effect of selected rumen fauna on fibrolytic enzyme activities, bacterial mass, fibre disappearance and fermentation pattern in sheep
 
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2003-01-02
 
J. Anim. Feed Sci. 2003;12(1):45–64
 
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ABSTRACT
Three Polish Merino wethers, fed hay and ground barley, were defaunated and then faunated with Eudiplodinium maggii only; with Eudiplodinium maggii and Entodinium caudatum or with both opryoscolecids and Dasytricha ruminantium. The total number of ciliates varied in the range of 16-403 x 103/g rumen content depending on the experimental period. Total bacterial contributed to 12.7-19.6% DM of rumen digesta. The bacterial matter in two of three sheep was negatively influenced by Entodinium caudatum and Dasytricha ruminantium while the effect of Eudiplodinium maggii was positive. The establishment of ophryoscolecid ciliates in the rumen resulted in an increase in both CMC-ase and xylanase activities (P<0.01), while the appearance of Dasytricha ruminantium was accompanied by a decrease in xylanolytic activity. A positive relationship was found between the numbers of Eudiplodinium maggii and both CMC-ase and xylanase activities (P<0.05) irrespective of the presence or absence of other protozoa in the rumen. It was calculated that 8-38% of the activity of CMC-ase and 14-49% of xylanase in the rumen originated from Eudiplodinium maggii. The quantities of dry matter, ADF and NDF that disappeared from the rumen within 12 h after feeding were 0.62-0.83, 0.18-0.32 and 0.32-0.54 kg, respectively. Establishment of Eudiplodinium maggii in the rumen was accompanied by increased disappearance of DM, ADF and NDF in only one of three animals. A decrease in disappearance of DM, ADF and NDF was observed following the establishment of Dasytricha ruminantium in all three animals. The concentration of acetic, propionic and butyric acids was within the ranges of 5.25-7.94, 1.29-2.34 and 0.75-1.39 mM/100 ml of rumen fluid, respectively. Acetate and propionate were the lowest in the sheep with the established population of Eudiplodinium maggii, whereas butyrate, in animals with all three ciliate species in the rumen. The highest concentration of acetic acid was observed following establishment of both entodiniomorphid species, while that of butyrate, in sheep inoculated with Eudiplodinium maggii. The production rate of acetate, propionate and butyrate was 21.3-24.5, 4.7-6.6 and 2.8-5.6 μM/g rumen contents/h, respectively. Production of acetic acid was not influenced by protozoa. An increase in the production rate of butyrate at 4 h after feeding was found after establishment of Eudipoldinium maggii in the rumen. Appearance of Entodinium caudatum and Dasytricha ruminantium decreased the production of butyrate at 4 h after and propionate just before feeding, respectively.
 
CITATIONS (9):
1.
Can fungal zoospores be the source of energy for the rumen protozoa Eudiplodinium maggii?
Renata Miltko, Grzegorz Bełżecki, Barbara Kowalik, Tadeusz Michałowski
Anaerobe
 
2.
The effect of rumen ciliates on chitinolytic activity, chitin content and the number of fungal zoospores in the rumen fluid of sheep
Renata Miltko, Grzegorz Bełżecki, Andrzej Herman, Barbara Kowalik, Jacek Skomiał
Archives of Animal Nutrition
 
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Chitinolytic activity of the sheep rumen ciliate Diploplastron affine
G. Bełżecki, R. Miltko, T. Michałowski, J. Šimůnek, J. Kopečný
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In vitro methane formation and carbohydrate fermentation by rumen microbes as influenced by selected rumen ciliate species
Johanna O. Zeitz, Michael Kreuzer, Carla R. Soliva
European Journal of Protistology
 
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Response of the Rumen Microbiota of Sika Deer (Cervus nippon) Fed Different Concentrations of Tannin Rich Plants
Zhipeng Li, André-Denis G. Wright, Hanlu Liu, Zhongyuan Fan, Fuhe Yang, Zhigang Zhang, Guangyu Li, Garret Suen
PLOS ONE
 
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Effect of Entodinium caudatum on starch intake and glycogen formation by Eudiplodinium maggii in the rumen and reticulum
Grzegorz Bełżecki, Neil R. McEwan, Barbara Kowalik, Tadeusz Michałowski, Renata Miltko
European Journal of Protistology
 
7.
Effect of the rumen ciliatesEntodinium caudatum, Epidinium ecaudatumandEudiplodinium maggii, and combinations thereof, on ruminal fermentation and total tract digestion in sheep
Johanna O. Zeitz, Sergej L. Amelchanka, Tadeusz Michałowski, Krzysztof Wereszka, Leo Meile, Sonja Hartnack, Michael Kreuzer, Carla R. Soliva
Archives of Animal Nutrition
 
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Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves
Li-feng DONG, Jun-nan MA, Yan TU, Qi-yu DIAO
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9.
Rapeseed and linseed oil supplementation affects hydrolytic activities in the rumen of sheep
Małgorzata Majewska, Renata Miltko, Agata Krawczyńska, Grzegorz Bełżecki, Barbara Kowalik
Livestock Science
 
ISSN:1230-1388