ORIGINAL PAPER
The effect of a commercial microbial phytase preparation on the in vitro release of phosphorus and amino acids from selected plant feedstuffs supplemented with free amino acids
 
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1
Institute of Food, Nutrition and Human Health, Palmerston North, New Zealand
2
DSM Nutritional Products Asia Pacific Pte Ltd., Singapore
3
Riddet Centre, Massey University, Palmerston North, New Zealand
CORRESPONDING AUTHOR
S. M. Rutherfurd   

Institute of Food, Nutrition and Human Health, Palmerston North, New Zealand
Publication date: 2004-10-25
 
J. Anim. Feed Sci. 2004;13(4):677–690
 
KEYWORDS
ABSTRACT
A commercially available microbial phytase was investigated with the aim of firstly, determining its effectiveness in releasing phosphate from phytate and secondly, investigating the possible binding of supplemented amino acids to phytate and the effectiveness of microbial phytase in releasing bound amino acids. Feedstuffs with added synthetic amino acids were incubated in the presence or absence of microbial phytase and the resulting concentrations of free phosphate and free amino acids were determined. The mean dephosphorylation of phytate over all feedstuffs and reaction conditions used was 0.40. Dephosphorylation ranged from 0 for wheat pollard to 0.84 for cottonseed meal depending on the feedstuff and the reactions conditions used. Incubation in the absence and presence of microbial phytase resulted in a recovery of supplemented amino acids of between 0.37 to 1.15 (mean = 0.84) and 0.49 to 1.17 (mean = 0.89), respectively, depending on the amino acid, feedstuff and reaction conditions employed.
 
CITATIONS (4):
1.
Effect of microbial phytase on phytate P degradation and apparent digestibility of total P and Ca throughout the gastrointestinal tract of the growing pig1
S. M. Rutherfurd, T. K. Chung, P. J. Moughan
Journal of Animal Science
 
2.
High doses of phytase on growth performance and apparent ileal amino acid digestibility of broilers fed diets with graded concentrations of digestible lysine
Carrie L Walk,, Savaram Venkata Rama Rao
Journal of Animal Science
 
3.
Effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus corn-soybean meal diet for broilers
S.M. Rutherfurd, T.K. Chung, D.V. Thomas, M.L. Zou, P.J. Moughan
Poultry Science
 
4.
Does estimation methods affect on phosphorus equivalence value of phytase for layer and broiler chickens?
Azam Yousefi, Mojtaba Zaghari, Amir Karamzadeh-Dehaghani
 
ISSN:1230-1388