0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

The development potential of bovine embryo co-culture with Vero and Vero/BRL cells

A. M. Duszewska 1  ,  
J. Wojdan 2,  
W. Gawron 2,  
B. Waś 1,  
 
1
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Department of Experimental Embryology, Jastrzębiec, 05-552 Wólka Kosowska, Poland
2
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Department of Cattle Genetics and Breeding, Jastrzębiec, 05-552 Wólka Kosowska, Poland
J. Anim. Feed Sci. 2007;16(3):370–379
Publish date: 2007-09-06
KEYWORDS
ABSTRACT
The objective of this study was to evaluate the effect of two bovine embryo co-culture systems on calving rates. The embryos were co-cultured with Vero or Vero/BRL cells (mixed co-culture) until the blastocyst stage. A higher percentage of blastocysts was observed on Vero/BRL cells, 42.85%, compared with 30.41% on Vero cells (P≤0.05). The blastocysts from Vero/BRL and Vero cells were transferred to recipients. A higher rate of calving was obtained after transfer of embryos co-cultured with Vero cells (37.50%), compared with Vero/BRL cells (13.16%) (P≤0.01). A greater loss of pregnancy after transfer of embryos co-cultured with Vero/BRL cells was observed between 35 and 65 days. All calves were born naturally, healthy, and with normal weight. Vero cells better support cattle prenatal development than Vero/BRL cells. Probably Vero/BRL cells lead to epigenetic modifications that are responsible for early foetal resorption.
CORRESPONDING AUTHOR
A. M. Duszewska   
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Department of Experimental Embryology, Jastrzębiec, 05-552 Wólka Kosowska, Poland
 
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ISSN:1230-1388