ORIGINAL PAPER
Sequence polymorphism of exon 17 of the ryanodine receptor gene (ryr1) in the Canidae
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K. Nuc 2
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1
Department of Pig Breeding and Production, Laboratory of Immunogenetics, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland,
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Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland,
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Department of Human Genetics, Polish Academy of Sciences, Struszyńska 32, 60-479 Poznań, Poland
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Department of Animal Breeding and Genetics, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland,
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K. Marcinkowski University of Medical Science, III Chair and Department of Surgery, Przybyszewskiego 49, 60-355 Poznań, Poland
Publication date: 2000-10-04
 
J. Anim. Feed Sci. 2000;9(4):721–726
 
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ABSTRACT
Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and sequencing analysis were used to study the ryanodine receptor gene (ryr1) of the blue fox (Alopex lagopus), wolf (Canis lupus), and domestic dog (Canis familiaris). PCR-amplified ryr1-gene-specific fragments were cloned in a pGem5 Zf(-) plasmid. Double-stranded templates were sequenced by the Sanger dideoxy chain termination method, using Cy-5' labelled primers. We detected G→C transversion (position 1836) in the wolf ryr1 gene, and T→C transition (position 1857) in the blue fox (these mutations do not alter the respective amino acid sequences) and a C→T transition (position 1846) in the ryr1 of the blue fox that changes the amino acid from serine to proline. We did not detect the 1843C→T mutation in the Canidae ryr1 gene.
ISSN:1230-1388