ORIGINAL PAPER
Sequence polymorphism of exon 17 of the ryanodine receptor gene (ryr1) in the Canidae
P. Gronek 1,   K. Nuc 2,   D. Napierała 3,   A. Pławski 3,   M. Zając 4,   T. Banasiewicz 5,   R. Słomski 2, 3
 
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1
Department of Pig Breeding and Production, Laboratory of Immunogenetics, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland,
2
Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland,
3
Department of Human Genetics, Polish Academy of Sciences, Struszyńska 32, 60-479 Poznań, Poland
4
Department of Animal Breeding and Genetics, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland,
5
K. Marcinkowski University of Medical Science, III Chair and Department of Surgery, Przybyszewskiego 49, 60-355 Poznań, Poland
Publication date: 2000-10-04
 
J. Anim. Feed Sci. 2000;9(4):721–726
 
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ABSTRACT
Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and sequencing analysis were used to study the ryanodine receptor gene (ryr1) of the blue fox (Alopex lagopus), wolf (Canis lupus), and domestic dog (Canis familiaris). PCR-amplified ryr1-gene-specific fragments were cloned in a pGem5 Zf(-) plasmid. Double-stranded templates were sequenced by the Sanger dideoxy chain termination method, using Cy-5' labelled primers. We detected G→C transversion (position 1836) in the wolf ryr1 gene, and T→C transition (position 1857) in the blue fox (these mutations do not alter the respective amino acid sequences) and a C→T transition (position 1846) in the ryr1 of the blue fox that changes the amino acid from serine to proline. We did not detect the 1843C→T mutation in the Canidae ryr1 gene.
ISSN:1230-1388