ORIGINAL PAPER
Polymorphism in the 5’flanking region of the myostatin gene affects myostatin and TGF-β1 expression in bovine skeletal muscle
 
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1
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Nowoursynowska 159, 02-787 Warsaw, Poland
2
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Wólka Kosowska, Poland
CORRESPONDING AUTHOR
T. Motyl   

Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Nowoursynowska 159, 02-787 Warsaw, Poland
Publication date: 2006-07-05
 
J. Anim. Feed Sci. 2006;15(3):381–391
 
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ABSTRACT
Myostatin (GDF-8) is a key protein responsible for skeletal muscle growth and development so mutations in the mstn gene can have major economic and breeding consequences. The aim of the present study was to investigate polymorphism in the 5’flanking region of the mstn gene and its possible influence on the myostatin level in skeletal muscles of Polish Black-and-White bulls. The relation between expression of myostatin and another member of the TGF-β superfamily, TGF-β1, was also examined. We uncovered polymorphism in the 5’flanking region of the mstn gene: G/C substitution at position -7828 (relative to translation start codon ATG). The most frequent genotype was GC (43.6%), followed by genotypes CC (34.2%) and GG (22.2%). The concentration of the active form of myostatin (26 kDa) in M. semitendinosus of homozygotes (CC) was the lowest, whereas the expression of this cytokine in heterozygotes was the highest. The changes in mstn expression did not, however, influence average carcass traits (weight of valuable cuts and weight of lean in valuable cuts). The pattern of differences in the TGF-β1 concentration corresponded to that observed for myostatin.
 
CITATIONS (4):
1.
Comparison of skeletal muscle transcriptional profiles in dairy and beef breeds bulls
T. Sadkowski, M. Jank, L. Zwierzchowski, J. Oprządek, T. Motyl
Journal of Applied Genetics
 
2.
Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical gamma-oryzanol
K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. Sadkowski
Genes & Nutrition
 
3.
Gene expression profiling in skeletal muscle of Holstein-Friesian bulls with single-nucleotide polymorphism in the myostatin gene 5’-flanking region
Tomasz Sadkowski, Michał Jank, Lech Zwierzchowski, Eulalia Siadkowska, Jolanta Oprządek, Tomasz Motyl
Journal of Applied Genetics
 
4.
Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation
Carla Grade, Carolina Mantovani, Lúcia Alvares
Journal of Animal Science and Biotechnology
 
ISSN:1230-1388