ORIGINAL PAPER
Polymerase chain reaction detection of caecal bacteria in case of preventive application of Enterococcus faecium EK13 against Salmonella enterica subsp. Enteritidis in chickens
R. Herich 1  
,   A. Lauková 2,   V. Strompfová 2,   V. Revajová 1,   M. Levkut 1,   J. Pistl 3
 
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1
Institute of Pathology, University of Veterinary Medicine, Komenského 73, 040 01 Košice, Slovak Republic
2
Institute of Animal Physiology, Slovak Academy of Sciences, Pri hati 10, 040 01 Košice, Slovak Republic
3
Department of Microbiology, University of Veterinary Medicine, Komenského 73, 040 01 Košice, Slovak Republic
CORRESPONDING AUTHOR
R. Herich   

Institute of Pathology, University of Veterinary Medicine, Komenského 73, 040 01 Košice, Slovak Republic
Publication date: 2005-01-31
 
J. Anim. Feed Sci. 2005;14(1):161–170
 
KEYWORDS
ABSTRACT
The polymerase chain reaction (PCR) was used in combination with microbiology methods for identifying faecal bacteria. The changes were monitor in the bacterial flora of chicken’s caecum after preventive peroral application of Enterococcus faecium EK13 and consecutive infection with Salmonella enterica subsp. Enteritidis.The preventive treatment with Enteroccocus faecium EK13, reduced the numbers of S. enterica subsp. Enteritidis in chicken excreta after 5 days post infection (pi) and in the caecum after 7 days pi. The PCR products 243 bp for Sallmonella spp. and 550 bp for Ent. faecium were amplified. The detection of Salmonella spp. required only simple boiling of the samples. On the contrary, for reliable detection of Ent. faecium, DNA isolation with commercial available kit was required. The high sensitivity of PCR was confirmed and the expected specific DNA fragments could be amplified from dilutions containing ten to one CFU (Colony Forming Units).
 
CITATIONS (2):
1.
IgA gene expression and quantification of cecal IgA+, IgM+, and CD4+ cells in chickens treated with EFAL41 and infected with Salmonella Enteritidis
Katarína Bobíková, Viera Revajová, Viera Karaffová, Mária Levkutová, Mikuláš Levkut
Acta Histochemica
 
2.
Expression of cytokines in chicken peripheral mononuclear blood cells (PMBCs) exposed to probiotic strains and Salmonella Enteritidis
Eva Husáková, Viera Spišáková, Róbert Herich, Martina Kolesárová, Dominika Stašová, Mária Levkutová, Mikuláš Levkut
Acta Veterinaria Brno
 
ISSN:1230-1388