1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

New polymorphisms and expression of the porcine ghrelin (GHRL) gene in different pig breeds

K. Ropka-Molik 1  ,  
M. Oczkowicz 1,  
M. Różycki 2,  
J. Romanek 3,  
 
1
National Research Institute of Animal Production, Laboratory of Genomics, 32-083 Balice, Poland
2
National Research Institute of Animal Production, Department of Genetics and Animal Breeding, 32-083 Balice, Poland
3
National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
4
National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, 32-083 Balice, Poland
J. Anim. Feed Sci. 2011;20(2):186–199
Publication date: 2011-06-18
KEYWORDS
ABSTRACT
Growth and development traits are economically important in animal production, especially in pig breeding. Therefore, the porcine GHRL gene is considered as a candidate gene responsible for growth rate and body weight. The aim of our study was to identify new polymorphisms in the GHRL gene in pig. Ten novel single nucleotide polymorphisms (SNP’s) were detected: four substitutions in exons, four in introns and two mutations in promoter region. We evaluated the GHRL mRNA abundance in porcine stomachs (fundus ventriculis) and ghrelin protein concentration in plasma in three breeds: Polish Landrace, Polish Large White and Pietrain. The results showed that transcript abundance of GHRL gene was significantly higher in Polish Landrace than in other two breeds (P<0.05). The mutation c.-93A>G located in the promoter region affected expression of the GHRL gene. The AA genotype animals showed a significantly (P<0.05) higher expression level when compared to AG genotype animals.
CORRESPONDING AUTHOR
K. Ropka-Molik   
National Research Institute of Animal Production, Laboratory of Genomics, 32-083 Balice, Poland
 
CITATIONS (2):
1. Association of Ghrelin Gene Polymorphisms with Fattening Traits and Feed Intake in Pig: A Preliminary Study
Mirosław Tyra, Katarzyna Ropka-Molik, Katarzyna Piórkowska, Maria Oczkowicz, Magdalena Szyndler-Nędza, Martyna Małopolska
Animals
2. Ghrelin gene single nucleotide polymorphisms and their effects on Nile tilapia (Oreochromis niloticus) growth
Binglin Chen, Wei Xiao, Zhiying Zou, Jinglin Zhu, Dayu Li, Jie Yu, Hong Yang
Aquaculture Reports
ISSN:1230-1388