0.857
IF5
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Q3
JCR
0.92
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ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Effects of dietary fish oil on NFκB gene expression and related signaling in spleen of chickens stimulated with lipopolysaccharide

X. Yang 1, 2,  
Xi He 2,  
B. Zhang 2,  
Y. Yang 2,  
J. Yuan 2,  
Y. Guo 2  
 
1
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Northwest A&F University, YangLing, 712100, P.R. China
2
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University, Beijing, 100094, P.R. China
J. Anim. Feed Sci. 2011;20(2):206–223
Publish date: 2011-06-18
KEYWORDS
ABSTRACT
The study was conducted to investigate the effects of fish oil and maize oil on nuclear factor kappa B (NFκB) gene expression and the downstream pathways of intracellular signaling in spleen of chickens after lipopolysaccharide (LPS) stimulation. Two hundred eighty eight chickens were assigned in a 2×2 factorial design. Factors were dietary fat type (4.5% maize oil or 4.5% fish oil) and immunological stimulation (LPS or saline). LPS increased levels of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) of spleen in chickens after the second LPS stimulation on 28 d of age. Fish oil alleviated the increase of EPA and DHA in spleen of chickens after LPS stimulation at 27 d of age. Fish oil supplementation decreased prostaglandin 2 (PGE2) production and the activity of cyclooxygenase 2 (COX2) after LPS stimulation. LPS stimulation increased the activity of phospholipase C (PLC) in spleen of chickens. And fish oil inhibited activity of PLC in spleen of chickens stimulated by LPS. Meanwhile fish oil decreased the production of inositol triphosphate (IP3) in spleen of chickens stimulated by LPS. Fish oil alleviated the mRNA abundance elevation of nuclear factor kappa B (NFκB) after LPS stimulation. These results showed that fish oil down-regulated the production of IP3 and PGE2 through inhibiting the activity of PLC and COX2 in spleen of chickens, respectively. The results of NFκB gene expression suggested fish oil might alleviate immune stress at the level of transcription.
CORRESPONDING AUTHOR
Y. Guo   
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University, Beijing, 100094, P.R. China
 
CITATIONS (2):
1. Mechanistic study of signalling pathways in intestinal B lymphocytes using eicosapentaenoic and docosahexaenoic acid in broiler chickens
Xin Yang, Yibing Wang, Junhu Yao, Xiaojun Yang
Food and Agricultural Immunology
2. The relationship between dietary fat sources and immune response in poultry and pigs: An updated review
S. Swiatkiewicz, A. Arczewska-Wlosek, D. Jozefiak
Livestock Science
ISSN:1230-1388