ORIGINAL PAPER
Effect of liquid storage on membrane integrity and
mitochondrial activity: a new diagnostic method of
evaluating boar sperm quality
| 1 | National Research Institute of Animal Production,
Department of Biotechnology of Animal Reproduction,
32-083 Balice, Poland |
CORRESPONDING AUTHOR
M. Trzcińska
National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
Publication date: 2008-06-24
J. Anim. Feed Sci. 2008;17(3):372–380
KEYWORDS
ABSTRACT
The aim of this study was to determine sperm membrane integrity and mitochondrial activity, to
quantify possible apoptosis, and to investigate relationship between these parameters. Apoptotic-like
changes were evaluated using a combination of YO-PRO-1/PI staining, and changes in mitochondrial
membrane potential were evaluated using JC-1. Gel-free ejaculates collected from five boars were
extended in BTS diluent and stored for six days at 15-17°C. The fluorescence analysis was repeated
on the first, third and sixth day of semen storage. Motility was also assessed on each day of the
experiment. The percentage of apoptotic sperm detected by YO-PRO-1/PI staining inconsiderable
decreased, while number of necrotic sperm increased during six days storage. The percentage of
spermatozoa with mitochondrial membrane potential (ΔΨm) dysfunction increased and reached the
highest level at day six of semen storage. The ΔΨm analysis by JC-1 revealed that mitochondrial
function was highly correlated with both sperm motility (r=0.936) and apoptosis/necrosis evaluated
by the YO-PRO-1/PI assay (r=0.960).
CITATIONS (11):
1.
Apoptotic-like changes in the spermatozoa of fresh and stored boar semen and the quality of embryos produced in vivo
M. Trzcińska, M. Bryła, Z. Smorąg
Animal Reproduction Science
M. Trzcińska, M. Bryła, Z. Smorąg
Animal Reproduction Science
2.
Quality and fertilizing capacity of boar spermatozoa during liquid storage in extender supplemented with different antibiotics
Magdalena Bryła, Monika Trzcińska
Animal Reproduction Science
Magdalena Bryła, Monika Trzcińska
Animal Reproduction Science
3.
Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos
M. Bryła, M. Trzcińska, J. Wieczorek, R. Słomski, Z. Smorąg
Animal Reproduction Science
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Alexander V. Makarevich, Eliska Spalekova, Lucia Olexikova, Elena Kubovicova, Zdenka Hegedusova
Zygote
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Assessment of plasma membrane and chromatin structure of sperm from transgenic and non-transgenic boars
M. Trzcińska, M. Bryła, M. Bochenek, R. Słomski, Z. Smorąg
Theriogenology
M. Trzcińska, M. Bryła, M. Bochenek, R. Słomski, Z. Smorąg
Theriogenology
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Polish Journal of Veterinary Sciences
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Annals of Animal Science
Monika Trzcińska, Magdalena Bryła
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