ORIGINAL PAPER
Effect of liquid storage on membrane integrity and mitochondrial activity: a new diagnostic method of evaluating boar sperm quality
M. Trzcińska 1  
,   M. Bryła 1,   Z. Smorąg 1
 
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National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
CORRESPONDING AUTHOR
M. Trzcińska   

National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
Publication date: 2008-06-24
 
J. Anim. Feed Sci. 2008;17(3):372–380
 
KEYWORDS
ABSTRACT
The aim of this study was to determine sperm membrane integrity and mitochondrial activity, to quantify possible apoptosis, and to investigate relationship between these parameters. Apoptotic-like changes were evaluated using a combination of YO-PRO-1/PI staining, and changes in mitochondrial membrane potential were evaluated using JC-1. Gel-free ejaculates collected from five boars were extended in BTS diluent and stored for six days at 15-17°C. The fluorescence analysis was repeated on the first, third and sixth day of semen storage. Motility was also assessed on each day of the experiment. The percentage of apoptotic sperm detected by YO-PRO-1/PI staining inconsiderable decreased, while number of necrotic sperm increased during six days storage. The percentage of spermatozoa with mitochondrial membrane potential (ΔΨm) dysfunction increased and reached the highest level at day six of semen storage. The ΔΨm analysis by JC-1 revealed that mitochondrial function was highly correlated with both sperm motility (r=0.936) and apoptosis/necrosis evaluated by the YO-PRO-1/PI assay (r=0.960).
 
CITATIONS (11):
1.
Apoptotic-like changes in the spermatozoa of fresh and stored boar semen and the quality of embryos produced in vivo
M. Trzcińska, M. Bryła, Z. Smorąg
Animal Reproduction Science
 
2.
Quality and fertilizing capacity of boar spermatozoa during liquid storage in extender supplemented with different antibiotics
Magdalena Bryła, Monika Trzcińska
Animal Reproduction Science
 
3.
Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos
M. Bryła, M. Trzcińska, J. Wieczorek, R. Słomski, Z. Smorąg
Animal Reproduction Science
 
4.
Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa
Alexander V. Makarevich, Eliska Spalekova, Lucia Olexikova, Elena Kubovicova, Zdenka Hegedusova
Zygote
 
5.
Assessment of plasma membrane and chromatin structure of sperm from transgenic and non-transgenic boars
M. Trzcińska, M. Bryła, M. Bochenek, R. Słomski, Z. Smorąg
Theriogenology
 
6.
Effects of the platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen (AI) with reduced sperm motility
M. Lecewicz, W. Kordan, A. Majewska, S. Kamiński, A. Dziekońska, K. Mietelska
Polish Journal of Veterinary Sciences
 
7.
Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C
A. Dziekońska, L. Fraser, A. Majewska, M. Lecewicz, Ł. Zasiadczyk, W. Kordan
Polish Journal of Veterinary Sciences
 
8.
Effect of Diluent and Storage Time on Sperm Characteristics of Rooster Insemination Doses
J. Vasicek, L. Kuzelova, B. Kulikova, P. Chrenek
Avian Biology Research
 
9.
Effect of thymosin β on maturation of pig oocytes and quality of in vitro produced embryos
K. Poniedziałek-Kempny, B. Gajda, I. Rajska, L. Gajda, Z. Smorąg
Journal of Animal and Feed Sciences
 
10.
Use of Novel Methods to Assess Seasonal Differences in the Quality of Boar Semen Stored Up to 7 Days at 17°C
Barbara Szczęśniak-Fabiańczyk, Michał Bochenek, Piotr Gogol, Monika Trzcińska, Magdalena Bryła, Zdzisław Smorąg
Annals of Animal Science
 
11.
A new sperm selection criterion for cryopreservation of boar semen
Monika Trzcińska, Magdalena Bryła
Annals of Animal Science
 
ISSN:1230-1388