SHORT COMMUNICATION
An NMR-based metabolomic approach to assess
metabolism in splanchnic tissues of steers
1
Department of Food Science, Danish Institute of Agricultural Sciences,
Box 50, DK-8830 Tjele, Denmark
2
Department of Animal Nutrition and Physiology,
Danish Institute of Agricultural Sciences,
Box 50, DK-8830 Tjele, Denmark
3
Interdisciplinary Nanoscience Center (iNANO), Department of Chemistry,
University of Aarhus,
Langelandsgade 140, DK-8000 Aarhus C, Denmark
4
Department of Animal Sciences, University of Kentucky,
Lexington, KY 40546, USA
Publication date: 2004-08-30
Corresponding author
N. B. Kristensen
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Box 50, DK-8830 Tjele, Denmark
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Box 50, DK-8830 Tjele, Denmark
J. Anim. Feed Sci. 2004;13(Suppl. 1):295-298
KEYWORDS
ABSTRACT
1H NMR spectra were acquired on plasma samples obtained from the mesenteric artery, hepatic
portal vein and the hepatic vein of steers (n=6) to demonstrate a NMR-based metabolomic approach
to study splanchnic metabolism. The metabolic differences between the different plasma samples
(arterial, portal, hepatic) were examined by partial least square regressions (PLSR) on the obtained
spectra. These analyses revealed spectral differences that could be ascribed to changes in the level
of propionate, β-hydroxybutyrate, lactate, acetate, n-butyrate and glucose, which was supported by
biochemical analyses.
CITATIONS (3):
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3.
Metabonomics in Pharmaceutical Discovery and Development
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