A note on comparison of two extraction methods used to quantify C18 fatty acids in feed and digesta of ruminants

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ORIGINAL PAPER

A note on comparison of two extraction methods used to quantify C18 fatty acids in feed and digesta of ruminants

A. Cieślak ¹

,

A. Machmüller ²

,

M. Szumacher-Strabel ¹

,

M. R. L. Scheeder ^2,3

1

University of Life Sciences, Department of Animal Nutrition and Feed Management, Wołyńska 33, 60-637 Poznań, Poland

2

ETH Zurich, Institute of Animal Sciences Universitaetstr. 2, CH-8092 Zurich, Switzerland

3

Swiss College of Agriculture, Laenggasse 85, CH-3052 Zollikofen, Switzerland

Publication date: 2009-03-20

Corresponding author

A. Cieślak

University of Life Sciences, Department of Animal Nutrition and Feed Management, Wołyńska 33, 60-637 Poznań, Poland

J. Anim. Feed Sci. 2009;18(2):362-367

DOI: https://doi.org/10.22358/jafs/66400/2009

KEYWORDS

C18 fatty acids

dichloromethane

extraction method

ABSTRACT

The aim of the present study was to compare two methods of fatty acid determination and quantification, differing in the kind of solvent used. Our hypothesis stated that diethyl ether can replace dichloromethane for the extraction of fatty acids, facilitate the analysis, and reduce health hazards. The analyses were conducted on feed and duodenal digesta samples from an experiment investigating the effects of feeding increasing amounts of either rape seed or linseed oils to sheep. Based on the obtained results, we conclude that the diethyl ether method seems to be more efficient for extraction of fatty acids from hydrolysed feed and digesta. Further investigations are needed to determine whether diethyl ether can replace dichloromethane for the extraction of fatty acids from other matrices.

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