ORIGINAL PAPER
A new set of endogenous reference genes for gene expression studies of porcine stomach
 
More details
Hide details
1
National Research Institute of Animal Production, Department of Genetics and Animal Breeding, 32-083 Balice, Poland
2
National Research Institute of Animal Production, Department of Immuno and Cytogenetics, 32-083 Balice, Poland
CORRESPONDING AUTHOR
M. Oczkowicz   

National Research Institute of Animal Production, Department of Genetics and Animal Breeding, 32-083 Balice, Poland
Publication date: 2010-11-26
 
J. Anim. Feed Sci. 2010;19(4):570–576
 
KEYWORDS
ABSTRACT
Gene expression analysis by Real-Time PCR requires careful selection of endogenous reference genes to obtain accurate results. We evaluated usefulness of six reference genes to expression studies of porcine stomach. We selected genes which were recently described as very stable (RPL27, RPS29, RPS13, OAZ1) and two commonly used housekeeping genes (GAPDH, ACTB). Our results indicate that OAZ1, RPS29 and RPL27 are more suitable reference genes than ACTB and GAPDH in expression studies of porcine stomach. In our study the most stable genes were OAZ1 (M=0.856), RPS29 (M=0.862) and RPL27 (M=0.892), while ACTB (M=1.03) and GAPDH (M=1.005) were less stable. RPS13 (M=1.913) appeared to be highly unstable. Set of three the most stable genes has been used to compare ghrelin (GHRL) expression in two different regions (diverticulum ventriculi and fundus ventriculi) of porcine stomach. Ghrelin mRNA was highly expressed in both regions of stomach, however level of GHRL mRNA was approximately 5-fold higher in fundus ventriculi than in diverticulum ventriculi.
 
CITATIONS (5):
1.
Selection of optimal reference genes for quantitative RT-PCR studies of boar spermatozoa cryopreservation
Changjun Zeng, Lian He, Wenpei Peng, Li Ding, Keyi Tang, Donghui Fang, Yan Zhang
Cryobiology
 
2.
The normalisation ofCAPNgene expression inM. pectoralis superficialisin broiler lines differing in growth rate and their relationship to breast muscle tenderness
K. Piórkowska, J. Nowak, K. Połtowicz
British Poultry Science
 
3.
Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues
Muhammad Jasim Uddin, Mehmet Ulas Cinar, Dawit Tesfaye, Christian Looft, Ernst Tholen, Karl Schellander
BMC Research Notes
 
4.
Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA
Mehmet Cinar, Mohammad Islam, Muhammad Uddin, Ernst Tholen, Dawit Tesfaye, Christian Looft, Karl Schellander
BMC Research Notes
 
5.
Evaluation of suitable reference genes for gene expression studies in porcine PBMCs in response to LPS and LTA
Mehmet Cinar, Mohammad Islam, Maren Pröll, Hakan Kocamis, Ernst Tholen, Dawit Tesfaye, Christian Looft, Karl Schellander, Muhammad Uddin
BMC Research Notes
 
ISSN:1230-1388