ORIGINAL PAPER
The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing
L. Stádník 1  
,   A. V. Makarevich 2, 3,   M. Doležalová 1,   E. Kubovičová 2, 3,   J. Beran 1,   Z. Hegedüšová 3,   J. Čítek 1,   R. Holásek 3,   R. Stupka 1
 
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1
Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Kamycka 129, 165 21 Prague 6 - Suchdol, Czech Republic
2
Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovak Republic
3
Institute for Cattle Breeding Ltd. Rapotin, Vyzkumniku 267, 788 13 Vikyrovice, Czech Republic
CORRESPONDING AUTHOR
L. Stádník   

Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Kamycka 129, 165 21 Prague 6 - Suchdol, Czech Republic
Publication date: 2014-11-27
 
J. Anim. Feed Sci. 2014;23(4):309–316
 
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ABSTRACT
The objective of this study was to evaluate effect of the season on the yield and viability of embryos recovered from superovulated and flushed Holstein cows (n = 56). The embryo recovery rate (ERR, %) was determined as the proportion of totally flushed embryos (TFE, n) to the number of corpora lutea detected. The embryos were evaluated as transferable (TRANS) or non-transferable and analysed for occurrence of apoptotic cells (T+, TUNELindex) as well as actin cytoskeleton quality (ACG). TFE and ERR were the highest during spring vs summer (149 embryos, 59.6% vs 20 embryos, 37%; P < 0.001), while they insignificantly differed in autumn (57 embryos, 48.3%). The autumn embryos presented a higher proportion of TRANS (P < 0.05, +10.56% vs spring; +18.94% vs summer). The occurrence of apoptosis in these embryos was lower (–1.38 T+ cells and –1.75% T-index; P < 0.05) and the percentage of the best ACG was higher (+35%; P < 0.05). These findings identified autumn as the best season for collection of high-quality bovine embryos.
ISSN:1230-1388