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ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing

L. Stádník 1  ,  
A. V. Makarevich 2, 3,  
E. Kubovičová 2, 3,  
J. Beran 1,  
J. Čítek 1,  
R. Holásek 3,  
 
1
Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Kamycka 129, 165 21 Prague 6 - Suchdol, Czech Republic
2
Animal Production Research Centre Nitra, 951 41 Lužianky near Nitra, Slovak Republic
3
Institute for Cattle Breeding Ltd. Rapotin, Vyzkumniku 267, 788 13 Vikyrovice, Czech Republic
J. Anim. Feed Sci. 2014;23(4):309–316
Publish date: 2014-11-27
KEYWORDS
ABSTRACT
The objective of this study was to evaluate effect of the season on the yield and viability of embryos recovered from superovulated and flushed Holstein cows (n = 56). The embryo recovery rate (ERR, %) was determined as the proportion of totally flushed embryos (TFE, n) to the number of corpora lutea detected. The embryos were evaluated as transferable (TRANS) or non-transferable and analysed for occurrence of apoptotic cells (T+, TUNELindex) as well as actin cytoskeleton quality (ACG). TFE and ERR were the highest during spring vs summer (149 embryos, 59.6% vs 20 embryos, 37%; P < 0.001), while they insignificantly differed in autumn (57 embryos, 48.3%). The autumn embryos presented a higher proportion of TRANS (P < 0.05, +10.56% vs spring; +18.94% vs summer). The occurrence of apoptosis in these embryos was lower (–1.38 T+ cells and –1.75% T-index; P < 0.05) and the percentage of the best ACG was higher (+35%; P < 0.05). These findings identified autumn as the best season for collection of high-quality bovine embryos.
CORRESPONDING AUTHOR
L. Stádník   
Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Kamycka 129, 165 21 Prague 6 - Suchdol, Czech Republic
ISSN:1230-1388