The utilization of methods based on protein and
DNA analysis for identification of animal-origin
components in feeds

M. Natonek; E. Stoła; A. Żyga; B. Rejduch

doi:10.22358/jafs/70306/2004

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Suppl. 2/2004 vol. 13

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The utilization of methods based on protein and DNA analysis for identification of animal-origin components in feeds

M. Natonek ¹

E. Stoła ¹

A. Żyga ¹

B. Rejduch ¹

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National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Immuno- and Cytogenetics, Krakowska 1, 32-083 Balice, Poland

Publication date: 2004-09-16

Corresponding author

M. Natonek

National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Immuno- and Cytogenetics, Krakowska 1, 32-083 Balice, Poland

J. Anim. Feed Sci. 2004;13(Suppl. 2):75-78

DOI: https://doi.org/10.22358/jafs/70306/2004

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KEYWORDS

BSE

meat-and-bone meal

PCR reaction

ELISA

ABSTRACT

The described methods are based on detecting protein and mtDNA in feeds. Analyses targeting protein were based on ELISA tests, those detecting mtDNA were conducted using PCR. The method based on analysing mtDNA permits identification of components of animal origin (porcine, ovine, bovine) and semi-quantitative estimation of DNA content. These characteristics make it suitable for routine control of feeds. ELISA assays are less efficient for such analyses.

CITATIONS (1):

Methane mitigation in ruminants: from microbe to the farm scale
C. Martin, D. P. Morgavi, M. Doreau
animal

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1230-1388

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