ORIGINAL PAPER
The effect of mixed bacterial inoculant on the microbial population
and aerobic stability of lucerne and maize balage
1
Lithuanian University of Health Sciences, Institute of Animal Science, R. Zebenkos 12, LT-82317 Baisogala, Radviliskis distr., Lithuania
2
Lithuanian University of Health Sciences, Faculty of Animal Husbandry Technology, Department of Animal Breeding and Nutrition, Tilzes 18, LT-47181 Kaunas, Lithuania
Publication date: 2019-12-27
Corresponding author
R. Stoškus
Lithuanian University of Health Sciences, Institute of Animal Science, R. Zebenkos 12, LT-82317 Baisogala, Radviliskis distr., Lithuania
Lithuanian University of Health Sciences, Institute of Animal Science, R. Zebenkos 12, LT-82317 Baisogala, Radviliskis distr., Lithuania
J. Anim. Feed Sci. 2019;28(4):383-391
KEYWORDS
TOPICS
ABSTRACT
Two forages, maize (Zea mays) and lucerne (Medicago sativa),
having opposite fermentability coefficient, were ensiled in big bales for 120 days
without and with application of a dual purpose bacterial inoculant containing
Lactococcus lactis and Lactobacillus buchneri at 1.5 × 105 cfu/g of fresh forage.
The experiments were performed using the random sampling method, where
each big bale was an experimental unit. The combination of each raw material
and the inoculant was replicated ten times. Inoculated maize and lucerne
silages had greater level of lactic acid (P < 0.01) and acetic acid (P < 0.01) as
well as reduced pH value (P < 0.01) than not inoculated ones. Application of
bacterial inoculant decreased yeast (P < 0.01) and mould (P < 0.01) counts and
increased lactic acid bacteria count (P < 0.01). Inoculant had a positive effect
on aerobic stability by delaying aerobic deterioration time. The inoculation of
maize and lucerne was effective at improving the fermentation aerobic stability
and decreasing yeast and mould growth.
REFERENCES (26)
1.
AOAC International, 2000. Official Methods of Analysis of AOAC International. 17th Edition. Gaithersburg, MD (USA).
2.
Auerbach H., Nadeau E., 2018. Biological and chemical additives maintain nutritive value of grass silage. In: K. Gerlach, K.H. Südekum (Editors). Proceedings of the XVIII International Silage Conference. Bonn (Germany), pp. 220–221.
3.
Borreani G., Tabacco E., Schmidt R.J., Holmes B.J., Muck R.E., 2018. Factors affecting dry matter and quality losses in silages. J. Dairy Sci. 101, 3952–3979, https://doi.org/10.3168/jds.20....
4.
Copani G., Milora N., Bryan K.A., Nielsen N. G., Witt K. L., 2018. Fermentation and aerobic stability of grass and grass-legume silages ensiled. In: K. Gerlach, K.H. Südekum (Editors). Proceedings of the XVIII International Silage Conference. Bonn (Germany). pp. 286–287.
5.
Copani G., Nielsen N.G., Bryan K.A., Vrotniakiene V., Jatkauskas J., Witt K.L., 2017. Effects of a dual strain inoculant on alfalfa silage fermentation and aerobic stability through 32 days of ensiling. In: L.G. Nussio, D.O. De Sousa, V.C. Gritti, G.G. De Souza Salvati, W.P. Dos Santos, P.A.R. Salvo (Editors). Proceedings of the V International Symposium on Forage Quality and Conservation. Piracicaba, Sao Paulo (Brazil).
6.
DLG, 2000. DLG guidelines for the testing of silage additives for compliance with DLG quality label requirements. DLG Verlags-GmbH. Frankfurt a.M. (Germany).
7.
Đorđević S., Mandić V., Stanojević D., Jovanović-Ljesković N., 2017. Effects of Lactobacillus plantarum inoculants on maize silage quality. Biotechnol. Anim. Husbandry 33, 115–125, https://doi.org/10.2298/bah170....
8.
EFSA, 2006. Opinion of the Panel on additives and products or substances used in animal feed (FEEDAP) for the establishment of guidelines on the assessment of safety and efficacy of silage additives, on a request from the Commission under Article 7(5) of Regulation (EC) No 1831/2003. EFSA-Q-2004-088. EFSA J. 374, 1–115.
9.
Gallo A., Bernardes T.F., Copani G., Fortunati P., Giuberti G., Bruschi S., Bryan K.A., Nielsen N.G., Witt K.L., Masoero F., 2018. Effect of inoculation with Lactobacillus buchneri LB1819 and Lactococcus lactis O224 on fermentation and mycotoxin production in maize silage compacted at different densities. Anim. Feed Sci. Technol. 246, 36–45, https://doi.org/10.1016/j.anif....
10.
ISO, 1998. ISO method 15214:1998. Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of mesophilic lactic acid bacteria – Colony-count technique at 30 degrees C.
11.
ISO, 2008. ISO method 21527-1:2008. Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of yeasts and moulds – Part 1: Colony count technique in products with water activity greater than 0.95.
12.
Jatkauskas J., Vrotniakiene V., Ohlsson C., Lund B., 2013. The effects of three silage inoculants on aerobic stability in grass, clover-grass, lucerne and maize silages. Agric. Food Sci. 22, 137–144, https://doi.org/10.23986/afsci....
13.
Jatkauskas J., Vrotniakiene V., Stoškus R., 2018. Variations in fermentation, bacterial population and aerobic stability in maize silage. Zemdirbyste-Agric. 105, 377–382, https://doi.org/10.13080/z-a.2....
14.
Johnson J.R. (Editor), 2017. Techniques to increase silage stability and starch availability and the effects of heat stress abatement systems on reducing heat load in dairy cattle. PhD Dissertation. Kansas State University (USA).
15.
Juškienė V., Jatkauskas J., Bliznikas S., Švirmickas G., Uchockis V., Vrotniakienė V., 2014. Quality assessment of feedingstuffs in livestock productivity controls in dairy cattle. Final Report (in Lithuanian: Pašarų kokybės įvertinimas pieninių galvijų bandose, kuriose vykdoma produktyvumo kontrolė. Galutinė ataskaita). Lithuanian University of Health Sciences, Baisogala (Lithuania), pp. 61.
16.
Kung L., Shaver R.D., Grant R.J., Schmidt R.J., 2018. Silage review: Interpretation of chemical, microbial and organoleptic components of silages. J. Dairy Sci. 101, 4020–4033, https://doi.org/10.3168/jds.20....
17.
Li X., Xu W., Yang J., Zhao H., Pan C., Ding X., Zhang Y., 2016. Effects of applying lactic acid bacteria to the fermentation on a mixture of corn steep liquor and air-dried rice straw. Anim. Nutr. 2, 229–233, https://doi.org/10.1016/j.anin....
18.
Mogodiniyai Kasmaei K., Rustas B.-O., Spörndly R., Udén P., 2013. Prediction models of silage fermentation products on crop composition under strict anaerobic conditions: A metaanalysis. J. Dairy Sci. 96, 6644–6649, https://doi.org/10.3168/jds.20....
19.
Ogunade I.M., Arriola K.G., Jiang Y., Driver J.P., Staples C.R., Adesogan A.T., 2016. Effects of 3 sequestering agents on milk aflatoxin M1 concentration and the performance and immune status of dairy cows fed diets artificially contaminated with aflatoxin B.., J. Dairy Sci. 99, 6263–6273, https://doi.org/10.3168/jds.20....
20.
Playne M.J., McDonald P., 1966. The buffering constituents of herbage and of silage. J. Sci. Food Agric. 17, 264–268, https://doi.org/10.1002/jsfa.2....
21.
Queiroz O.C.M., Adesogan A.T., Arriola K.G., Queiroz M.F.S., 2012. Effect of a dual-purpose inoculant on the quality and nutrient losses from maize silage produced in farm-scale silos. J. Dairy Sci. 95, 3354–3362, https://doi.org/10.3168/jds.20....
22.
Queiroz O.C.M., Ogunade I.M., Weinberg Z., Adesogan A.T., 2018. Silage review: Foodborne pathogens in silage and their mitigation by silage additives. J. Dairy Sci. 101, 4132–4142, https://doi.org/10.3168/jds.20....
23.
Tyrolová Y., Bartoň L., Loučka R., 2017. Effects of biological and chemical additives on fermentation progress in maize silage. Czech J. Anim. Sci. 62, 306–312, https://doi.org/10.17221/67/20....
24.
Weissbach F., 1996. New developments in crop conservation. In: Proceedings of the 11th International Silage Conference. Aberystwyth, Wales (UK), pp. 11–25.
25.
Weissbach F., Strubelt C., 2008. Correcting the dry matter content of maize silages as a substrate for biogas production. Landtechnik 63, 210–211.
26.
Witt K.L., Jatkauskas J., Vrotniakiene V., Voss M.T., 2015. Effects of a novel dual purpose silage additive on aerobic stability and fermentation characteristics of whole crop maize silage after a short time of ensiling, In: L.G. Nussio, D.O. De Sousa, V.C. Gritti, G.G. De Souza Salvati, W.P. Dos Santos, P.A.R. Salvo (Editors). Proceedings of the XVII International Silage Conference. Piracicaba, Sao Paulo (Brazil), pp. 82–83.
CITATIONS (1):
1.
The prevalence of mycotoxins and their relation to nutrient composition of maize and grass silage
Eimantas Venslovas, Lauksme Merkeviciute-Venslove, Audrone Mankeviciene, Yuliia Kochiieru, Alvyra Slepetiene, Jurgita Ceseviciene
Zemdirbyste-Agriculture
Eimantas Venslovas, Lauksme Merkeviciute-Venslove, Audrone Mankeviciene, Yuliia Kochiieru, Alvyra Slepetiene, Jurgita Ceseviciene
Zemdirbyste-Agriculture
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