ORIGINAL PAPER
The effect of LPS on LH release and gene expression of LH-β, GnRH-R and TLR4 in the anterior pituitary of follicular phase ewes – an in vitro study
 
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
CORRESPONDING AUTHOR
K. Haziak   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
A. P. Herman   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2013-06-07
 
J. Anim. Feed Sci. 2013;22(2):97–105
 
KEYWORDS
ABSTRACT
The study was designed to determine the in vitro effect of bacterial endotoxin – lipopolysaccharide (LPS) – on luteinizing hormone (LH) secretion from anterior pituitary (AP) explants collected from saline-treated ‘healthy’ and LPS-treated ewes in the follicular phase. In the AP explants, the expression of LH-β, gonadotropin-releasing hormone receptor (GnRH-R) and Toll-like receptor 4 (TLR4) genes was also assayed. It was found that explants incubated alone with LPS, as well as together with LPS-binding protein (LBP), abolished the stimulatory effect of GnRH on LH release. LPS added without LBP did not suppress LH-β gene expression in ‘healthy’ APs, however. Moreover, LBP intensified the inhibitory effect of LPS on gene expression in ‘healthy’ APs. These results show that LPS is a potent negative modulator of LH secretion and suggest that its direct action on the pituitary gland could be one of the mechanisms via which an immune/inflammatory challenge inhibits the reproductive process.
 
CITATIONS (8):
1.
Involvement of prolactin in the meloxicam-dependent inflammatory response of the gonadotropic axis to prolonged lipopolysaccharide treatment in anoestrous ewes
Andrzej P. Herman, Agata Krawczyńska, Joanna Bochenek, Hanna Antushevich, Anna Herman, Dorota Tomaszewska-Zaremba
Reproduction, Fertility and Development
 
2.
Effects of Central Injection of Anti-LPS Antibody and Blockade of TLR4 on GnRH/LH Secretion during Immunological Stress in Anestrous Ewes
Karolina Haziak, Andrzej Przemysław Herman, Dorota Tomaszewska-Zaremba
Mediators of Inflammation
 
3.
How does bacterial endotoxin influence gonadoliberin/gonadotropins secretion and action?
D. Tomaszewska-Zaremba, A. Herman, K. Haziak
Journal of Animal and Feed Sciences
 
4.
Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study
Karolina Wojtulewicz, Dorota Tomaszewska-Zaremba, Andrzej Herman
Molecules
 
5.
Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
Karolina Haziak, Andrzej Przemysław Herman, Karolina Wojtulewicz, Bartosz Pawlina, Kamila Paczesna, Joanna Bochenek, Dorota Tomaszewska-Zaremba
Journal of Animal Science and Biotechnology
 
6.
Inflammation and LPS-Binding Protein Enable the Stimulatory Effect of Endotoxin on Prolactin Secretion in the Ovine Anterior Pituitary: Ex Vivo Study
Dorota Tomaszewska-Zaremba, Karolina Haziak, Monika Tomczyk, Andrzej P. Herman
Mediators of Inflammation
 
7.
Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle
Andrzej Herman, Janina Skipor, Agata Krawczyńska, Joanna Bochenek, Karolina Wojtulewicz, Bartosz Pawlina, Hanna Antushevich, Anna Herman, Dorota Tomaszewska-Zaremba
International Journal of Molecular Sciences
 
8.
The Influence of Anandamide on the Anterior Pituitary Hormone Secretion in Ewes—Ex Vivo Study
Dorota Tomaszewska-Zaremba, Karolina Wojtulewicz, Kamila Paczesna, Monika Tomczyk, Katarzyna Biernacka, Joanna Bochenek, Andrzej Herman
Animals
 
ISSN:1230-1388