1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

The effect of LPS on LH release and gene expression of LH-β, GnRH-R and TLR4 in the anterior pituitary of follicular phase ewes – an in vitro study

K. Haziak 1  ,  
A. P. Herman 1  ,  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2013;22(2):97–105
Publication date: 2013-06-07
KEYWORDS
ABSTRACT
The study was designed to determine the in vitro effect of bacterial endotoxin – lipopolysaccharide (LPS) – on luteinizing hormone (LH) secretion from anterior pituitary (AP) explants collected from saline-treated ‘healthy’ and LPS-treated ewes in the follicular phase. In the AP explants, the expression of LH-β, gonadotropin-releasing hormone receptor (GnRH-R) and Toll-like receptor 4 (TLR4) genes was also assayed. It was found that explants incubated alone with LPS, as well as together with LPS-binding protein (LBP), abolished the stimulatory effect of GnRH on LH release. LPS added without LBP did not suppress LH-β gene expression in ‘healthy’ APs, however. Moreover, LBP intensified the inhibitory effect of LPS on gene expression in ‘healthy’ APs. These results show that LPS is a potent negative modulator of LH secretion and suggest that its direct action on the pituitary gland could be one of the mechanisms via which an immune/inflammatory challenge inhibits the reproductive process.
CORRESPONDING AUTHOR
K. Haziak   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
A. P. Herman   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
CITATIONS (8):
1. Involvement of prolactin in the meloxicam-dependent inflammatory response of the gonadotropic axis to prolonged lipopolysaccharide treatment in anoestrous ewes
Andrzej P. Herman, Agata Krawczyńska, Joanna Bochenek, Hanna Antushevich, Anna Herman, Dorota Tomaszewska-Zaremba
Reproduction, Fertility and Development
2. Effects of Central Injection of Anti-LPS Antibody and Blockade of TLR4 on GnRH/LH Secretion during Immunological Stress in Anestrous Ewes
Karolina Haziak, Andrzej Przemysław Herman, Dorota Tomaszewska-Zaremba
Mediators of Inflammation
3. How does bacterial endotoxin influence gonadoliberin/gonadotropins secretion and action?
D. Tomaszewska-Zaremba, A. Herman, K. Haziak
Journal of Animal and Feed Sciences
4. Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study
Karolina Wojtulewicz, Dorota Tomaszewska-Zaremba, Andrzej Herman
Molecules
5. Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
Karolina Haziak, Andrzej Przemysław Herman, Karolina Wojtulewicz, Bartosz Pawlina, Kamila Paczesna, Joanna Bochenek, Dorota Tomaszewska-Zaremba
Journal of Animal Science and Biotechnology
6. Inflammation and LPS-Binding Protein Enable the Stimulatory Effect of Endotoxin on Prolactin Secretion in the Ovine Anterior Pituitary: Ex Vivo Study
Dorota Tomaszewska-Zaremba, Karolina Haziak, Monika Tomczyk, Andrzej P. Herman
Mediators of Inflammation
7. Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle
Andrzej Herman, Janina Skipor, Agata Krawczyńska, Joanna Bochenek, Karolina Wojtulewicz, Bartosz Pawlina, Hanna Antushevich, Anna Herman, Dorota Tomaszewska-Zaremba
International Journal of Molecular Sciences
8. The Influence of Anandamide on the Anterior Pituitary Hormone Secretion in Ewes—Ex Vivo Study
Dorota Tomaszewska-Zaremba, Karolina Wojtulewicz, Kamila Paczesna, Monika Tomczyk, Katarzyna Biernacka, Joanna Bochenek, Andrzej Herman
Animals
ISSN:1230-1388