The objective of this study was to evaluate the effect of adding resveratrol (RSV) to a hypercholesterolaemic diet on the lipid profile, activity of antioxidant enzymes and their mRNA gene expression, as well as lipid concentration in selected organs of Wistar rats. Animals were divided into four groups and fed experimental diets for 8 weeks. The negative control group (NC) was fed AIN-93G diet. The positive control group (PC) received a hypercholesterolaemic diet (AIN-93G + 0.1% cholesterol, 7% butter). The other groups were fed PC diets supplemented with 0.05% and 0.1% RSV, respectively. An addition to the PC diet RSV (0.05 and 0.1%, respectively) significantly decreased concentration of total cholesterol (TC) and LDL-cholesterol in serum of rats compared to the PC group. The triacylglycerols (TAG) level was significantly lower in serum of rats fed with 0.05% of RSV in comparison to other experimental groups. Activities of antioxidant enzymes and their mRNA gene expression were not affected by RSV. In conclusion, relatively high doses of RSV were sufficient in lowering TC and LDL-cholesterol, and TAG and oxidative stress without involving naturally occurring mechanisms in rats.