ORIGINAL PAPER
Oocyte maturation in the presence of randomly pooled follicular fluid increases bovine blastocyst yield in vitro
 
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Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Wolka Kosowska, Poland
CORRESPONDING AUTHOR
J. A. Modliński   

Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Wolka Kosowska, Poland
Publication date: 2005-08-04
 
J. Anim. Feed Sci. 2005;14(3):501–512
 
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ABSTRACT
The objective of the study was to examine preimplantation development of the zygotes derived from bovine oocytes matured in the presence of follicular fluid (bFF) randomly pooled from large (>10 mm in diameter) highly vascularised ovarian follicles originating from slaughter-house ovaries. The maturation medium was supplemented with 20% bFF. The controls comprised 20% bFF+10% FCS, and 10% FCS. The use of three separate batches of bFF have shown that the rates of cleavage (78-87%) and blastocyst formation (about 40%) obtained after bFF-supplemented maturation are similar or higher than those in the FCS control. In another experiment morphological selection of Grade I oocytes was performed after maturation or insemination. Selection of Grade I matured oocytes increased cleavage rate (to 85-91%) and blastocyst yield (to 43%). When Grade I inseminated oocytes were selected, then 92-93% of zygotes divided, and 47% reached blastocyst stage. Blastocyst rates were higher after bFF-supplemented maturation than after FCS-supplemented one, both in Grade I matured (43.0 as opposed to 40.7%) and Grade I inseminated (47.3 against 45.8%) oocytes. The majority of all zygotes underwent the frst cleavage division between 27 and 30 hpi. Zygotes originating from oocytes matured in the presence of bFF or bFF+FCS were found dividing until 48 hpi whereas those obtained from control oocytes no longer cleaved after 33 hpi.In conclusion, oocyte maturation in the presence of bovine bFF randomly pooled from large follicles of slaughter-house ovaries supports as high developmental capacity of presumed zygotes as does the FCS-control.
 
CITATIONS (3):
1.
Adult follicular fluid supplementation during in vitro maturation improves the developmental competence of prepubertal lamb oocytes
Hao Tian, Kexiong Liu, Yumei Zhang, Qi Qi, Chunxin Wang, Hong Guan, Fengxiang Yan, Jian Hou
Theriogenology
 
2.
Effects of Dietary Urea on timing of embryo cleavages and blood components in Mice
A Attaai
Veterinary World
 
3.
Do the Cytoplast and Nuclear Material of Germinal Vesicle Oocyte Support Developmental Competence Upon Reconstruction with Embryonic/Somatic Nucleus
Abd Mohammed, Saker Al-Suwaiegh, Tarek Al-Shaheen
Cellular Reprogramming
 
ISSN:1230-1388