1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
REVIEW PAPER
 
CC-BY 4.0
 
 

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

B. Gajda 1  ,  
 
1
National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
J. Anim. Feed Sci. 2009;18(3):371–387
Publication date: 2009-06-24
KEYWORDS
ABSTRACT
During the past few decades, significant progress in cryopreservation of mammalian oocytes and embryos has been observed. Transfer of cryopreserved embryos or oocytes resulted in live offspring in at least 25 species. So far, two major methods have been used for oocyte and embryo cryopreservation: conventional slow-rate freezing and vitrification. This review summarizes the progress in cryopreservation of mammalian oocytes and embryos that has been achieved by modifying oocyte/embryo susceptibility to cryopreservation or vitrification technology through such techniques as solid-surface vitrification, cryoloop, microdrop, cryotop, electron microscopy grids, nylon mesh, open pulled straw method or removal of lipids, addition of antifreeze protein or cytoskeleton-stabilizing agents, cholesterol or liposomes, centrifugation prior to cryopreservation or application of high hydrostatic pressure. In conclusion, the vitrification method opened new perspectives in cryopreservation of embryos and oocytes, both for in vitro fertilized and somatic nuclear transfer. Authors believe that new cryopreservation procedures which modify the susceptibility of oocytes and embryos to cryopreservation will gain importance as a major tool in mammalian gamete and embryo cryopreservation.
CORRESPONDING AUTHOR
B. Gajda   
National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
 
CITATIONS (3):
1. New technique to quantify the lipid composition of lipid droplets in porcine oocytes and pre-implantation embryos using Nile Red fluorescent probe
Marek Romek, Barbara Gajda, Ewa Krzysztofowicz, Mariusz Kepczynski, Zdzisław Smorag
Theriogenology
2. Structural and functional evolution of chitinase-like proteins from plants
Pooja Kesari, Dipak Narhari Patil, Pramod Kumar, Shailly Tomar, Ashwani Kumar Sharma, Pravindra Kumar
PROTEOMICS
3. Goat oocytes quality after vitrification using difference of glycerol concentration and exposure time
S Wahjuningsih, G Ciptadi, M Ihsan, A Putri, H Karima
IOP Conference Series: Earth and Environmental Science
ISSN:1230-1388