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1.7
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MNiSW
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ICV
REVIEW PAPER
 
CC-BY 4.0
 
 

Obtaining farm animal embryos in vitro

A. M. Duszewska 1  ,  
Ł. Rąpała 1,  
P. Trzeciak 1,  
S. Dąbrowski 1,  
 
1
Warsaw University of Life Sciences, Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Nowoursynowska 159, 02-776 Warsaw
2
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Experimental Embryology Department, Jastrzębiec, Postępu 1, 05-552 Magdalenka, Poland
J. Anim. Feed Sci. 2012;21(2):217–233
Publication date: 2012-06-18
KEYWORDS
ABSTRACT
This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.
CORRESPONDING AUTHOR
A. M. Duszewska   
Warsaw University of Life Sciences, Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Nowoursynowska 159, 02-776 Warsaw
 
CITATIONS (2):
1. Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species
AM Duszewska, P Gręda, M Baraniewicz, W Bielecki, W Niżański, A Partyka, M Tracz, Z Nowak, A Chełmońska-Soyta, W Olech
Reproduction in Domestic Animals
2. Influence of elevated temperature on bovine oviduct epithelial cells (BOECs)
Łukasz Rąpała, Rafał R. Starzyński, Piotr Z. Trzeciak, Sebastian Dąbrowski, Małgorzata Gajewska, Piotr Jurka, Roman Smolarczyk, Anna M. Duszewska, Peter J. Hansen
PLOS ONE
ISSN:1230-1388