1.054
IF5
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IF
Q3
JCR
1.7
CiteScore
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SJR
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MNiSW
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ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Obtaining calves after transfer of embryos microinjected with the human interferon alpha (IFNα) gene, pbLGIFN-GFPBsd

A. M. Duszewska 1, 2  ,  
D. Lipiński 3, 4,  
W. Gawron 5,  
A. Rynkowska 1,  
R. Słomski 3, 4,  
 
1
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Department of Experimental Embryology, Jastrzębiec, 05-552 Wólka Kosowska, Poland
2
Warsaw University of Life Sciences, Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Nowoursynowska 159, 02-776 Warsaw, Poland
3
Poznan University of Life Sciences, Department of Biochemistry and Biotechnology, Wołyńska 35, 60-637 Poznań, Poland
4
Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland
5
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Department of Animal Sciences, Jastrzębiec, 05-552 Wólka Kosowska, Poland
J. Anim. Feed Sci. 2008;17(4):500–509
Publication date: 2008-10-27
KEYWORDS
ABSTRACT
The objective of this study was to obtain calves with an integrated human interferon alpha (IFNα) gene. The transgene (pbLGIFN-GFPBsd) was introduced by microinjection into one of the zygote’s pronuclei. The transgene (pbLGIFN-GFPBsd) contained the human interferon alpha (IFNα) gene, bovine lactoglobulin promoter (bLG), gfp (green fluorescence protein) and blasticidin resistance gene (bsd). After microinjection of 2107 zygotes, the proportion developing to the blastocyst stage (10.4%) was lower than for non-injected embryos (39.9%; P<0.01). Of the 2107 that were microinjected, 76 were GFP-positive blastocysts (3.6%). Fifty-seven GFP-positive blastocysts and 31 blastocysts from non-injected embryos were transferred singly to recipients. A lower rate of calving was observed after transfer of GFP-positive blastocysts (19.3%) as compared with noninjected embryos (48.4%; P<0.05). All newborn calves were healthy and of normal weight. PCR analyses indicated that none of the calves obtained after transfer of GFP-positive blastocysts carried the human interferon alpha gene.
CORRESPONDING AUTHOR
A. M. Duszewska   
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Department of Experimental Embryology, Jastrzębiec, 05-552 Wólka Kosowska, Poland
ISSN:1230-1388