1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Methods of DNA isolation from poultry meal

 
1
Department of Animal Immuno- and Cytogenetics, National Research Institute of Animal Production, 30-083 Balice, Poland
J. Anim. Feed Sci. 2007;16(3):490–496
Publication date: 2007-09-06
KEYWORDS
ABSTRACT
Legal regulations concerning BSE prevention stipulate a total ban on the addition of animal meals to the feed of animals whose meat is intended for consumption (Council Decision 2000/766/ EC). Council Regulation 1774/2002/EC allows the use of animal meals in non-ruminant feeds on the condition that the meals contain no material originating from the same species as the animals fed. Poultry meals are an animal component whose use is allowed by the latter regulation. The standard method of animal component identification (using silica) was found to be effective for poultry and feather meals, but not for blood meal. Isolation of DNA from blood spots was unsuccessful. Only the use of a Sherlock kit enabled a PCR product to be obtained. The sensitivity of the identification method from poultry blood meal was shown to be 2%. Analysis of poultry meal, feather meal and blood meal contaminated with bovine meal shows that the methods used for isolating DNA were effective.
CORRESPONDING AUTHOR
M. Natonek-Wiśniewska   
Department of Animal Immuno- and Cytogenetics, National Research Institute of Animal Production, 30-083 Balice, Poland
ISSN:1230-1388