ORIGINAL PAPER
Ferrous bisglycinate increased iron transportation through DMT1 and PepT1 in pig intestinal epithelial cells compared with ferrous sulphate
Zh.-Ch. Liao 1,   W.-T. Guan 1, 2  
,   F. Chen 1,   D.-X. Hou 1,   Ch.-X. Wang 1,   Y.-T. Lv 1,   H.-Z. Qiao 1,   J. Chen 1,   J.-H. Han 1
 
More details
Hide details
1
South China Agricultural University, College of Animal Science, Guangzhou 510642, China
2
National Engineering Research Center for Breeding Swine Industry, Guangzhou, 510642, China
CORRESPONDING AUTHOR
W.-T. Guan   

South China Agricultural University, College of Animal Science, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, Guangzhou, 510642, China
Publication date: 2014-06-11
 
J. Anim. Feed Sci. 2014;23(2):153–159
 
KEYWORDS
ABSTRACT
To study the different mechanisms of absorption of diverse iron sources, pig intestinal epithelial cells (IPEC) were incubated with various concentrations of ferrous sulphate and ferrous bisglycinate for different times to determine the expression of divalent metal transporter (DMT1), peptide transporter (PepT1), ferritin, transferrin receptor (TfR), and neutral amino acid transporter (ASCT1 and ASCT2) using RT-PCR and Western blotting. In the ferrous bisglycinate groups, the expression of DMT1 protein was higher at 24 h and a concentration of 1 mmol · l–1 (P < 0.05) and expression of PepT1 protein was higher in all treatments (P < 0.05); expression of TfR and ferritin mRNA was higher at 36 h, 0.5 mmol · l–1 and 1 mmol · l–1; and 24 h, 1 mmol · l–1; 36 h, 0.5 mmol · l–1 (P < 0.05), respectively; ASCT1 and ASCT2 mRNA expression was higher at 36 h, 0.5 mmol · l–1 (P < 0.05). We conclude that the more effective absorption of ferrous bisglycinate is associated with higher expression of iron-related transporters in IPEC cells.
 
CITATIONS (5):
1.
Dosage Effect of Zinc Glycine Chelate on Zinc Metabolism and Gene Expression of Zinc Transporter in Intestinal Segments on Rat
Danping Huang, Qiaoling Hu, Shenglin Fang, Jie Feng
Biological Trace Element Research
 
2.
Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells
Xiaonan Yu, Lingjun Chen, Haoxuan Ding, Yang Zhao, Jie Feng
Nutrients
 
3.
Oral iron supplementation with Feralgine® in inflammatory bowel disease: a retrospective observational study
Marta Vernero, Valentina Boano, Davide Ribaldone, Rinaldo Pellicano, Marco Astegiano
Minerva Gastroenterologica e Dietologica
 
4.
Different Sources of Copper Effect on Intestinal Epithelial Cell: Toxicity, Oxidative Stress, and Metabolism
Runxian Li, Yang Wen, Gang Lin, Chengzhen Meng, Pingli He, Fenglai Wang
Metabolites
 
5.
Iron Deficiency Anemia Refractory to Conventional Therapy but Responsive to Feralgine® in a Young Woman with Celiac Disease
Valentina Talarico, Laura Giancotti, Roberto Miniero, Marco Bertini
International Medical Case Reports Journal
 
ISSN:1230-1388