0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Ferrous bisglycinate increased iron transportation through DMT1 and PepT1 in pig intestinal epithelial cells compared with ferrous sulphate

Zh.-Ch. Liao 1,  
W.-T. Guan 1, 2  ,  
F. Chen 1,  
D.-X. Hou 1,  
Ch.-X. Wang 1,  
Y.-T. Lv 1,  
H.-Z. Qiao 1,  
J. Chen 1,  
 
1
South China Agricultural University, College of Animal Science, Guangzhou 510642, China
2
National Engineering Research Center for Breeding Swine Industry, Guangzhou, 510642, China
J. Anim. Feed Sci. 2014;23(2):153–159
Publish date: 2014-06-11
KEYWORDS
ABSTRACT
To study the different mechanisms of absorption of diverse iron sources, pig intestinal epithelial cells (IPEC) were incubated with various concentrations of ferrous sulphate and ferrous bisglycinate for different times to determine the expression of divalent metal transporter (DMT1), peptide transporter (PepT1), ferritin, transferrin receptor (TfR), and neutral amino acid transporter (ASCT1 and ASCT2) using RT-PCR and Western blotting. In the ferrous bisglycinate groups, the expression of DMT1 protein was higher at 24 h and a concentration of 1 mmol · l–1 (P < 0.05) and expression of PepT1 protein was higher in all treatments (P < 0.05); expression of TfR and ferritin mRNA was higher at 36 h, 0.5 mmol · l–1 and 1 mmol · l–1; and 24 h, 1 mmol · l–1; 36 h, 0.5 mmol · l–1 (P < 0.05), respectively; ASCT1 and ASCT2 mRNA expression was higher at 36 h, 0.5 mmol · l–1 (P < 0.05). We conclude that the more effective absorption of ferrous bisglycinate is associated with higher expression of iron-related transporters in IPEC cells.
CORRESPONDING AUTHOR
W.-T. Guan   
South China Agricultural University, College of Animal Science, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, Guangzhou, 510642, China
 
CITATIONS (2):
1. Dosage Effect of Zinc Glycine Chelate on Zinc Metabolism and Gene Expression of Zinc Transporter in Intestinal Segments on Rat
Danping Huang, Qiaoling Hu, Shenglin Fang, Jie Feng
Biological Trace Element Research
2. Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells
Xiaonan Yu, Lingjun Chen, Haoxuan Ding, Yang Zhao, Jie Feng
Nutrients
ISSN:1230-1388