ORIGINAL PAPER
Effect of pidotimod on growth performance, immune function,
intestinal epithelial barriers and microbiota of piglets
1
Zhejiang University, College of Animal Sciences, Institute of Animal Nutrition and Feed Sciences, Key Laboratory of Molecular
Animal Nutrition of the Ministry of Education, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention
and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, Zhejiang Province, China
2
Department of General Surgery, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch),
Hangzhou 311700, Zhejiang Province, China
Publication date: 2021-03-15
Corresponding author
L. Fu
Zhejiang University, College of Animal Sciences, Institute of Animal Nutrition and Feed Sciences, Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, Zhejiang Province, China
Zhejiang University, College of Animal Sciences, Institute of Animal Nutrition and Feed Sciences, Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, Zhejiang Province, China
W. Li
Zhejiang University, College of Animal Sciences, Institute of Animal Nutrition and Feed Sciences, Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, Zhejiang Province, China
Zhejiang University, College of Animal Sciences, Institute of Animal Nutrition and Feed Sciences, Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, Zhejiang Province, China
J. Anim. Feed Sci. 2021;30(1):64-75
KEYWORDS
TOPICS
ABSTRACT
Pidotimod (PTD) is a synthetic immunomodulatory dipeptide
widely used for immune regulation. This study aimed to investigate the effect
of PTD on growth performance, immune function, intestinal epithelial barriers
and microbiota in piglets. In total, 120 piglets with a weight of 25.26 ± 5.96 kg
and genetic makeup were selected and randomly divided into 4 groups with
3 replicates per group and 10 piglets per replicate. All piglets were weighed,
and from 3 piglets from each replicate (9 piglets per group) tissues were
collected. Piglets were fed for 44 days basal diet alone or supplemented with
50, 100 or 200 mg/kg PTD. It was found that the PTD addition increased the
average daily weight gain of piglets (P < 0.05 and P < 0.01 for dose 50 and
100 mg/kg, respectively). So, for further studies only dose 50 mg/kg PTD
was used. It was found that such dose decreased serum levels of interleukin
(IL)-6 (P < 0.05) and interferon (IFN)-γ (P < 0.01), but increased antiinflammatory cytokine IL-10 level (P < 0.05). Transmission electron micrographs
further revealed that supplementation with PTD at a dose of 50 mg/kg
enhanced intercellular connectivity, characterized by longer tight junction (TJ)
proteins, thicker adhesive tapes (AB) and desmosomes (D) in the jejunum.
This phenomenon was validated by the up-regulated protein expression of
occludin and ZO-1 in the jejunum. Furthermore, supplementation with PTD
at a dose of 50 mg/kg altered the α diversity of intestinal microbiota in piglets
(Observed_species, Chao1, PD_whole_tree, Ace). Particularly, the abundance
of Spirochaetes, Verrucomicrobia, Succinispira and Treponema was decreased,
but that of Lactobacillus and Collinsella was increased. Overall, dietary
supplementation with 50 mg/kg PTD improves the growth performance of
piglets by enhancing immune function, intestinal epithelial barrier and regulating
microbiota composition.
ACKNOWLEDGEMENTS
The present work was supported by the
grants from the planning subject of ‘the Twelfth
Five-year-plan’ in National Science and Technology
for the Rural Development in China (grant no. 2013BAD10B03). The authors thank Baikui Wang
for the microbial analysis of the manuscript.
CONFLICT OF INTEREST
The authors declare that there is no conflict of
interests.
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