Some progestogens as anabolic steroids can be illegally used for increasing the efficiency of
animal food production. The aim of the study was to develop convenient screening method against
progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was
developed and optimized. The ELISA method is class-specific for four relative progestogens and
the IC50 of the method was 3.0 μg/l for MPA. A simple feed pre-treatment method was developed for
analysis progestogen residues in feed. The limits of detection (LOD) were 2.0 μg/kg for MPA, 5.0
μg/kg for CMA, 4.6 μg/kg for MEGA and 3.9 μg/kg for HPA in the feed. The recovery was between
72.1 and 95.4% and the coefficients of variation (CV) were 7.2 to 15.8%. The results have showed
the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis
in feed.
CITATIONS(4):
1.
Development of determination of di-n-octyl phthalate (DOP) residue by an indirect enzyme-linked immunosorbent assay Hua Kuang, Liguang Xu, Gang Cui, Wei Ma, Chuanlai Xu Food and Agricultural Immunology
Development and application of one-step ELISA for the detection of neomycin in milk Naifeng Xu, Changlong Qu, Wei Ma, Liguang Xu, Liguang Xu, Liqang Liu, Hua Kuang, Chuanlai Xu Food and Agricultural Immunology