ORIGINAL PAPER
A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed
C.-F. Peng 1,   Y.-W. Chen 2,   H.-Q. Chen 1,   Ch.-L. Xu 1  
,   Z.-Y. Jin 1  
 
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1
School of Food Science and Technology, Southern Yangtze University, Wuxi 214122, P.R. China
2
Jiangsu Institute of Nuclear Medicine, Wuxi 214063, P.R. China
CORRESPONDING AUTHOR
Ch.-L. Xu   

School of Food Science and Technology, Southern Yangtze University, Wuxi 214122, P.R. China
Z.-Y. Jin   

School of Food Science and Technology, Southern Yangtze University, Wuxi 214122, P.R. China
Publication date: 2008-06-24
 
J. Anim. Feed Sci. 2008;17(3):434–441
 
KEYWORDS
ABSTRACT
Some progestogens as anabolic steroids can be illegally used for increasing the efficiency of animal food production. The aim of the study was to develop convenient screening method against progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was developed and optimized. The ELISA method is class-specific for four relative progestogens and the IC50 of the method was 3.0 μg/l for MPA. A simple feed pre-treatment method was developed for analysis progestogen residues in feed. The limits of detection (LOD) were 2.0 μg/kg for MPA, 5.0 μg/kg for CMA, 4.6 μg/kg for MEGA and 3.9 μg/kg for HPA in the feed. The recovery was between 72.1 and 95.4% and the coefficients of variation (CV) were 7.2 to 15.8%. The results have showed the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis in feed.
 
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ISSN:1230-1388