ORIGINAL PAPER
A rapid and sensitive enzyme-linked
immunosorbent assay (ELISA) method and
validation for progestogen multi-residues in feed
| 1 |
School of Food Science and Technology, Southern Yangtze University,
Wuxi 214122, P.R. China |
| 2 |
Jiangsu Institute of Nuclear Medicine,
Wuxi 214063, P.R. China |
J. Anim. Feed Sci. 2008;17(3):434–441
Publication date: 2008-06-24
KEYWORDS
ABSTRACT
Some progestogens as anabolic steroids can be illegally used for increasing the efficiency of
animal food production. The aim of the study was to develop convenient screening method against
progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was
developed and optimized. The ELISA method is class-specific for four relative progestogens and
the IC50 of the method was 3.0 μg/l for MPA. A simple feed pre-treatment method was developed for
analysis progestogen residues in feed. The limits of detection (LOD) were 2.0 μg/kg for MPA, 5.0
μg/kg for CMA, 4.6 μg/kg for MEGA and 3.9 μg/kg for HPA in the feed. The recovery was between
72.1 and 95.4% and the coefficients of variation (CV) were 7.2 to 15.8%. The results have showed
the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis
in feed.
CORRESPONDING AUTHOR
Ch.-L. Xu 
School of Food Science and Technology, Southern Yangtze University,
Wuxi 214122, P.R. China
Z.-Y. Jin
School of Food Science and Technology, Southern Yangtze University,
Wuxi 214122, P.R. China
CITATIONS (4):
| 1. |
Development of determination of di-n-octyl phthalate (DOP) residue by an indirect enzyme-linked immunosorbent assay
Hua Kuang, Liguang Xu, Gang Cui, Wei Ma, Chuanlai Xu
Food and Agricultural Immunology
|
| 2. |
Development and application of one-step ELISA for the detection of neomycin in milk
Naifeng Xu, Changlong Qu, Wei Ma, Liguang Xu, Liguang Xu, Liqang Liu, Hua Kuang, Chuanlai Xu
Food and Agricultural Immunology
|
| 3. |
Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue
Chi-Fang Peng, Xiao-Hui Duan, Qiu-Li Pan, Li-Qiang Liu, Feng Xue
Journal of Chemistry
|
| 4. |
Applications and recent developments of multi-analyte simultaneous analysis by enzyme-linked immunosorbent assays
Hongyan Zhang, Shuo Wang, Guozhen Fang
Journal of Immunological Methods
|
