TY - JOUR JO - Journal of Animal and Feed Sciences J2 - J. Anim. Feed Sci. SN - 1230-1388 VL - 26 IS - 2 PY - 2017 ID - Supapong2017 TI - In vitro fermentation, digestibility and methane production as influenced by Delonix regia seed meal containing tannins and saponins AB - The aim of the study was to evaluate the effect of supplementation of Delonix regia (DR) seed meal containing tannins and saponins on gas kinetics, ammonia-nitrogen (NH 3 -N) content, pH, methane (CH 4 ) production and dry matter (DM) digestibility using an in vitro gas production technique. The experimental design was completely randomized, and the dietary treatments included DR seed meal supplementation at levels of 0, 3.3, 5.0, 6.7, 8.3, 10, 11.7, 13.3, 15.0 and 16.7 mg DM added to 0.5 g of roughage and concentrate (70:30) mixture. The gas production was measured at several time points: 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h by a pressure transducer. The parameters of gas kinetics and cumulative gas production were not altered ( P > 0.05), except for gas production rate constant for the insoluble fraction which was the highest at 11.7 mg DR seed meal inclusion. CH 4 production and total protozoa counts linearly decreased with increasing DR seed meal levels ( P < 0.05). DR seed meal addition caused quadratic increase of in vitro DM digestibility with the highest value at 11.7 mg DR seed meal inclusion. No significant difference in volatile fatty acid profile ( P > 0.05) was stated between treatments except for propionic acid. In conclusion, supplementation of DR seed meal resulted in improved in vitro gas kinetics and DM digestibility up to 11.7 mg level, while CH 4 production was reduced linearly. The further in vivo studies are necessary to examine practical of DR seed meal usage in animal production. AU - Supapong, C. AU - Cherdthong, A. AU - Seankamsorn, A. AU - Khonkhaeng, B. AU - Wanapat, M. AU - Uriyapongson, S. AU - Gunun, N. AU - Gunun, P. AU - Chanjula, P. AU - Polyorach, S. SP - 123 EP - 130 DA - 2017 DO - 10.22358/jafs/73890/2017 UR - https://doi.org/10.22358/jafs/73890/2017 ER -