TY - JOUR JO - Journal of Animal and Feed Sciences J2 - J. Anim. Feed Sci. SN - 1230-1388 VL - 12 IS - 3 PY - 2003 ID - Malewski2003 TI - RT-PCR technique and its applications. State-of the-art AB - The reverse transcription polymerase chain reaction (RT-PCR) is one of the most sensitive methods for the detection and quantitation of mRNA. It is widely used for quantification of mRNA levels and is useful tool for basic research, agriculture, medicine and biotechnology. Introduction of Real-time technique significantly improves rapidity, sensitivity, specificity and reproducibility of RT-PCR method. Real-time PCR detects and quantifies nucleic acids even from live and dead pathogens or cells. This review discusses the background, advantages and limitations of conventional and Real-time RT-PCR methods for quantitation of gene expression and its application in basic, agricultural and biomedical research. AU - Malewski, T. AU - Malewska, A. AU - Rutkowski, R. SP - 403 EP - 416 DA - 2003 DO - 10.22358/jafs/67719/2003 UR - https://doi.org/10.22358/jafs/67719/2003 ER -