TY - JOUR JO - Journal of Animal and Feed Sciences J2 - J. Anim. Feed Sci. SN - 1230-1388 VL - 20 IS - 1 PY - 2011 ID - Duliński2011 TI - Comparison of high-performance ion chromatography technique with microbiological assay of myo-inositol in plant components of poultry feeds AB - An ion chromatography technique (HPAEC-PAD) was employed for determination of inositol in plant components of feeds as an alternative to a microbiological assay. The study included analysis of total, free and dialysable inositol released from samples by an in vitro procedure simulating digestion in the gastrointestinal tract of birds. Inositol was separated on the high-performance anion-exchange CarboPack MA1 column and monitored by the pulsed amperometric detection (PAD). As compared to the HPAEC-PAD technique, microbiological assay produced 2-3 fold lower values of total inositol, probably due to myo -inositol stereoselectivity of Saccharomyces cerevisiae ATCC 9080 analytical strain. The overall correlation coefficient was 0.989, 0.941 and 0.736 for total, free and dialysable inositol, respectively. Microbiological method of analysis rather than the HPAEC-PAD technique may be recommended for determination of dialysable myo -inositol released from feed samples during in vitro digestions that simulate gastrointestinal tract of birds. The HPAEC-PAD technique was more sensitive, convenient and suitable for total inositol determination. AU - Duliński, R. AU - Starzyńska-Janiszewska, A. AU - Stodolak, B. AU - Żyła, K. SP - 143 EP - 156 DA - 2011 DO - 10.22358/jafs/66165/2011 UR - https://doi.org/10.22358/jafs/66165/2011 ER -