0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

Xylanolytic activity of the rumen protozoan Diploplastron affine

K. Wereszka 1  ,  
C. J. Newbold 2,  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
The Institute of Rural Science, University of Wales, Aberystwyth Cerdigion SY23 3AL, United Kingdom
J. Anim. Feed Sci. 2006;15(Suppl. 1):43–46
Publish date: 2006-09-21
KEYWORDS
ABSTRACT
Cell free extract prepared from ciliates Diploplastron affine free from bacteria degraded xylan releasing reducing sugars at the rate of 117.8 ± 3.14 μM/mg protein/h. Two xylan degrading enzymes have been identified in protozoal cell free extract by a zymogram technique it was also found that one of the identified enzymes degraded xylan to xylooligosaccharides i.e. was of endoxylanase character. Screening of the Diploplastron affine cDNA library resulted in the identification of a xylanase encoding gene consisting of 1670 bp.
CORRESPONDING AUTHOR
K. Wereszka   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
ISSN:1230-1388