0.917
IF5
1.024
IF
Q2
JCR
0.90
CiteScore
0.385
SJR
Q2
SJR
20
MNiSW
142.18
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

The use of NIRS for prediction of protein content in residues after incubation in the rumen

J. Kański 1,  
 
1
Cracov Agricultural University, Department of Animal Nutrition, Al. Mickiewicza 24/28, 30-059 Kraków, Poland
J. Anim. Feed Sci. 2001;10(Suppl. 2):363–367
Publish date: 2001-06-28
KEYWORDS:
ABSTRACT:
The aim of the study was to investigate the possibility of using Near Infrared Reflectance Spectroscopy (NIRS) for determination of crude protein content in residues after incubation in the rumen. Samples of whole-crop grain silages (oats and triticale) of differing maturities (heading, milk, and milk-dough stages of growth) were incubated in the rumen of cannulated bulls for 2, 4, 8, 16, 24, 48, and 72 h. After incubation the bags were rinsed, washed and dried (45 °C, 48 h) and N content was determined chemically in the residues. The NIRS measurements were carried out on dried and ground (1 mm) silage samples. Samples were scanned in duplicate runs (a second run after rotation of the cup) over the range of 1445–2348 nm with a 19 filter-spectrophotometer InfraAlyser 450R (Technicon). Spectral data were recorded as log 1/R (R-reflectance) with IDAS PC software. Calibration equations were calculated with 3, 6 and 9 wavelengths (filters) for three sets: all samples (2 cereals x 3 stages of maturity x 7 incubation times x 3 animals; n = 126), A-subset (n = 72) for samples incubated for 2–16 h, B-subset for samples incubated for 24–72 h (n = 54). Based on a calibration for all samples (n = 126), crude protein content could be accurately predicted with R equal to 0.95, 0.96 and 0.97 and SEC equal to 0.46, 0.41 and 0.38, when 3, 6 or 9 wavelengths were considered in the equation, respectively. The precision of prediction increased as the number of wavelengths in the calibration equation increased.
 
CITATIONS (2):
1. Nutritive evaluation of herbage from permanent meadows by near-infrared reflectance spectroscopy: 2. Prediction of crude protein and dry matter degradability
Sonia Andrés, Alfredo Calleja, Secundino López, Ángel R Mantecón, F Javier Giráldez
Journal of the Science of Food and Agriculture
2. Nutritive Evaluation of Forages by near Infrared Reflectance Spectroscopy
Sonia Andrés, Ian Murray, Alfredo Calleja, F. Javier Giráldez
Journal of Near Infrared Spectroscopy
ISSN:1230-1388