CC-BY 4.0

Redox status, lipid peroxidation and protein oxidation levels in small ruminants

S. Cecchini 1  ,  
G. Piccione 2,  
C. Saoca 2,  
G. Giangrosso 3,  
A. R. Caputo 4,  
University of Basilicata, Department of Sciences, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
University of Messina, Department of Veterinary Science, Polo Universitario dell’Annunziata, 98168 Messina, Italy
Experimental Zooprophylaxis Institute of Sicily “A. Mirri”, 90121 Palermo, Italy
Indipendent Agronomist, 85050 Baragiano (PZ), Italy
J. Anim. Feed Sci. 2018;27(1):81–85
Publish date: 2018-02-28
Redox status, lipid peroxidation and protein oxidative damage in blood samples of healthy goats and sheep were evaluated to show any possible correlation with daily milk yield or milk components, and between oxidant parameters and biomarkers of protein and lipid damage. Blood and milk samples were collected from fifty Maltese goats and forty Valle del Belice sheep. Redox status was assessed analysing the total oxidant status (TOS), the total antioxidant capacity (TAC), together with the nitric oxide radical (NO) metabolite (NOx) content. Oxidative damage of lipids and proteins was analysed as thiobarbituric acid reactive substances (TBARS) and advanced oxidation protein products (AOPP), respectively. As for the comparison of the assessed oxidative stress (OS) parameters in the two species, only AOPP appeared significantly different (P < 0.01). While no correlation was observed between daily milk yield or milk components and the assessed OS parameters in both species, several correlations were shown among the assessed OS parameters, with differences appearing between the two species. In particular, TBARS levels were related to TOS in goats (P < 0.001) and to NOx in sheep (P < 0.001). Levels of AOPP were dependent on NOx concentrations in sheep (P < 0.05) but they were correlated neither with TOS nor NOx in goats (P > 0.05). So, the observed differences between two examined species seem to suggest that OS parameters are species-specific and a panel of different measurements based on various analytical methods should be considered to dutifully evaluate the OS.
S. Cecchini   
University of Basilicata, Department of Sciences, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy
1. Alberti A., Bolognini L., Macciantelli D., Caratelli M., 2000. The radical cation of N,N-diethyl-para-phenylendiamine: a possible indicator of oxidative stress in biological samples. Res. Chem. Intermed. 26, 253–267, https://doi.org/10.1163/156856700X00769.
2. Benzie I.F.F., Strain J.J., 1996. The ferric reducing ability of plasma(FRAP) as measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239, 70–76, https://doi.org/10.1006/abio.1996.0292.
3. Celi P., 2011. Biomarkers of oxidative stress in ruminant medicine. Immunopharmacol. Immunotoxicol. 33, 233–240, https://doi.org/10.3109/08923973.2010.514917.
4. Celi P., Di Trana A., Claps S., 2010. Effects of plane of nutrition on oxidative stress in goats during the peripartum period. Vet. J. 184, 95–99, https://doi.org/10.1016/j.tvjl.2009.01.014.
5. Celi P., Gabai G., 2015. Oxidant/antioxidant balance in animal nutrition and health: the role of protein oxidation. Front. Vet. Sci. 2, 48, https://doi.org/10.3389/fvets.2015.00048.
6. Czauderna M., Kowalczyk J., Marounek M., 2011. The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography. J. Chromatogr. B 879, 2251–2258, https://doi.org/10.1016/j.jchromb.2011.06.008.
7. Di Trana A., Bonanno A., Cecchini S., Giorgio D., Di Grigoli A., Claps S., 2015. Effects of Sulla forage (Sulla coronarium L.) on the oxidative status and milk polyphenol content in goats. J. Dairy Sci. 98, 37–46, https://doi.org/10.3168/jds.2014-8414.
8. Ermis B., Yildirim A., Örs R., Tastekin A., Ozkan B., Akcay F., 2005. Influence of smoking on serum and milk malondialdehyde, superoxide dismutase, glutathione peroxidase, and antioxidant potential levels in mothers at the postpartum seventh day. Biol. Trace Elem. Res. 105, 27–36, https://doi.org/10.1385/BTER:105:1-3:027.
9. Fazio F., Cecchini S., Saoca C., Caputo A.R., Lancellotti M., Piccione G., 2016. Relationship of some oxidative stress biomarkers in Jumper horses after regular training program. J. Equine Vet. Sci. 47, 20–24, https://doi.org/10.1016/j.jevs.2016.07.014.
10. Fazio F., Piccione G., Saoca C., Caputo A.R., Cecchini S., 2015. Assessment of oxidative stress in Flathead mullet (Mugilcephalus) and Gilthead sea bream (Sparus aurata). Vet. Med. 60, 691–695, https://doi.org/10.17221/8583-VETMED.
11. Gatellier P., Mercier Y., Renerre M., 2004. Effect of diet finishing mode (pasture or mixed diet) on antioxidant status of Charolais bovine meat. Meat Sci. 67, 385–394, https://doi.org/10.1016/j.meatsci.2003.11.009.
12. Giustarini D., Dalle-Donne I., Tsikas D., Rossi R., 2009. Oxidative stress and human diseases: origin, link, measurement, mechanisms, and biomarkers. Crit. Rev. Clin. Lab. Sci. 46, 241–281, https://doi.org/10.3109/10408360903142326.
13. Hanasand M., Omdal R., Norheim K.B., Gøransson L.G., Brede C., Jonsson G., 2012. Improved detection of advanced oxidation protein products in plasma. Clin. Chim. Acta 413, 901–906, https://doi.org/10.1016/j.cca.2012.01.038.
14. Konieczka P., Rozbicka-Wieczorek A.J., Więsyk E., Smulikowska S., Czauderna M., 2014. Improved derivatization of malondialdehyde with 2-thiobarbituric acid for evaluation of oxidative stress in selected tissues of chickens. J. Anim. Feed Sci. 23, 190–197, https://doi.org/10.22358/jafs/65709/2014.
15. Lykkesfeldt J., Svendsen O., 2007. Oxidants and antioxidants in disease: Oxidative stress in farm animals. Vet. J. 173, 502–511, https://doi.org/10.1016/j.tvjl.2006.06.005.
16. Miranda K.M., Espey M.G., Wink D.A., 2001. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5, 62–71, https://doi.org/10.1006/niox.2000.0319.
17. Nisar N.A., Sultana M., Waiz H.A., Para P.A., Dar S.A., 2013. Oxidative stress – threat to animal health and production. Int. J. Livest. Res. 3, 76–83.
18. Nordberg J., Arnér E.S.J., 2001. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic. Biol. Med. 31, 1287–1312, https://doi.org/10.1016/S0891-5849(01)00724-9.
19. Noyan T., Güler A., Şekeroğlu M.R., Kamaci M., 2006. Serum advanced oxidation protein products, myeloperoxidase and ascorbic acid in pre-eclampsia and eclampsia. Aust. N. Z. J. Obstet. Gynaecol. 46, 486–491, https://doi.org/10.1111/j.1479-828X.2006.00647.x.
20. Po E., Williams C., Muscatello G., Celi P., 2013. Assessment of oxidative stress biomarkers in exhaled breath condensate and blood of Thoroughbred foals. Vet. J. 196, 269–271, https://doi.org/10.1016/j.tvjl.2012.08.018.