Quantification of conjugated linoleic acid and other essential fatty acids in ovine meat, milk, fat, and intestinal digesta
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Department of Animal Nutrition and Feed Management, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland
Publication date: 2001-06-28
J. Anim. Feed Sci. 2001;10(Suppl. 2):385–392
Saturated fatty acids (FAs), positional and geometric isomers of mono-, di- and tri-unsaturated FAs containing eighteen carbon atoms were separated on two long HPLC RP-C18 columns. All FAs in ovine milk, meat, fat, and intestinal digesta were assayed after pre-column derivatization using UV detection at 256 nm. The content of conjugated linoleic acid (CLA) in processed samples can be determined by using simultaneous UV monitoring at 256 and 235 nm. The presented method is a simple, sensitive and selective analytical tool for routine quantification of FAs and CLA.
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