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ORIGINAL PAPER
 
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Molecular cloning, sequence characteristics analysis and tissue expression profiles of three novel genes RhoB, RhoF and RhoH from the Black-boned sheep (Ovis aries)

W. Li 1, 2,  
Zh. Chen 1,  
L. Yang 2,  
Y. Tan 2,  
Y. He 2,  
Q. Liu 2,  
G. Wu 2,  
B. Peng 2,  
H. Mao 2,  
W. Deng 2  
 
1
Department of Biomedical Engineering, School of Info-Physics and Geomatics Engineering, Central South University, Changsha 410083, P.R. China
2
Yunnan Provincial Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P.R. China
J. Anim. Feed Sci. 2009;18(2):271–282
Publish date: 2009-03-20
KEYWORDS:
ABSTRACT:
The complete coding sequences of three of the Black-boned sheep (Ovis aries) genes - RhoB, RhoF and RhoH were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) according to the conserved sequence information of the mouse or other mammals and known highly homologous sheep ESTs. The Black-boned sheep RhoB gene encodes a protein of 196 amino acids which contains the conserved putative RhoA_like domain and has high homology with the RhoB proteins of six species-cattle (100%), goat (100%), human (100%), pig (100%), mouse (100%) and chicken (97%). The Black-boned sheep RhoF gene encodes a protein of 215 amino acids that contains the conserved putative Rho4_like domain and has high homology with the RhoF proteins of five species-bovine (100%), human (94%), pig (93%), mouse (92%) and chicken (58%). The Black-boned sheep RhoH gene encodes a protein of 191 amino acids that contains the conserved putative Rho domain and has high homology with the RhoH proteins of four species-bovine (99%), human (96%), pig (96%) and mouse (96%). The phylogenetic tree analysis demonstrated that the Black-boned sheep RhoB, RhoF and RhoH proteins share a common ancestor. The tissue expression analysis indicated that the three genes were expressed in a range of tissues including small intestine, large intestine, liver, muscle, fat, lung and spleen. Our experiment is the first to provide the primary foundation for further insight into these three sheep genes.
CORRESPONDING AUTHOR:
W. Deng   
Yunnan Provincial Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P.R. China
 
CITATIONS (3):
1. Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from black-boned sheep (Ovis aries)
Y. D. He, D. D. Liu, D. M. Xi, L. Y. Yang, Y. W. Tan, Q. Liu, H. M. Mao, W. D. Deng
Molecular Biology
2. Molecular Cloning and Characteristics of thePSPH, snrpa1andTPM1Genes in Black-Boned Sheep (Ovis Aries)
Qiangfei Li, Dongmei Xi, Jing Leng, Xiao Gou, Huaming Mao, Weidong Deng
Biotechnology & Biotechnological Equipment
3. Molecular cloning, sequence identification and tissue expression profile of three novel genes Sfxn1, Snai2 and Cno from Black-boned sheep (Ovis aries)
Dongmei Xi, Yiduo He, Yongke Sun, Xiao Gou, Shuli Yang, Huaming Mao, Weidong Deng
Molecular Biology Reports
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ISSN:1230-1388