In vitro fermentation, digestibility and methane production as influenced by Delonix regia seed meal containing tannins and saponins
C. Supapong 1,   A. Cherdthong 1,2  
,   A. Seankamsorn 1,   B. Khonkhaeng 1,   M. Wanapat 1,2,   S. Uriyapongson 1,   N. Gunun 3,   P. Gunun 4,   P. Chanjula 5,   S. Polyorach 6
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Khon Kaen University, Faculty of Agriculture, Department of Animal Science, Khon Kaen 40002, Thailand
Khon Kaen University, Tropical Feed Resources Research and Development Centre (TROFREC), Khon Kaen 40002, Thailand
Udon Thani Rajabhat University, Faculty of Technology, Program in Animal Production Technology, Udon Thani 41000, Thailand
Rajamangala University of Technology-Isan, Sakon Nakhon Campus, Faculty of Natural Resources, Department of Animal Science, Phangkhon, Sakon Nakhon 47160, Thailand
Prince of Songkla University, Faculty of Natural Resources, Department of Animal Science, Songkhla 90112, Thailand
King Mongkut’s Institute of Technology Ladkrabang, Faculty of Agricultural Technology, Department of Animal Production Technology and Fisheries, Bangkok 10520, Thailand
A. Cherdthong   

Khon Kaen University, Faculty of Agriculture, Department of Animal Science, Khon Kaen 40002, Thailand
Publication date: 2017-06-06
J. Anim. Feed Sci. 2017;26(2):123–130
The aim of the study was to evaluate the effect of supplementation of Delonix regia (DR) seed meal containing tannins and saponins on gas kinetics, ammonia-nitrogen (NH3-N) content, pH, methane (CH4) production and dry matter (DM) digestibility using an in vitro gas production technique. The experimental design was completely randomized, and the dietary treatments included DR seed meal supplementation at levels of 0, 3.3, 5.0, 6.7, 8.3, 10, 11.7, 13.3, 15.0 and 16.7 mg DM added to 0.5 g of roughage and concentrate (70:30) mixture. The gas production was measured at several time points: 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h by a pressure transducer. The parameters of gas kinetics and cumulative gas production were not altered (P > 0.05), except for gas production rate constant for the insoluble fraction which was the highest at 11.7 mg DR seed meal inclusion. CH4 production and total protozoa counts linearly decreased with increasing DR seed meal levels (P < 0.05). DR seed meal addition caused quadratic increase of in vitro DM digestibility with the highest value at 11.7 mg DR seed meal inclusion. No significant difference in volatile fatty acid profile (P > 0.05) was stated between treatments except for propionic acid. In conclusion, supplementation of DR seed meal resulted in improved in vitro gas kinetics and DM digestibility up to 11.7 mg level, while CH4 production was reduced linearly. The further in vivo studies are necessary to examine practical of DR seed meal usage in animal production.
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