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Suppl. 1/2005 vol. 14
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SHORT COMMUNICATION
Improving the analysis of fatty acids using a combination of gas chromatography and Ag+ liquid chromatography
M. Czauderna 1
,
 
J. Kowalczyk 1
,
 
K. Korniluk 1
,
 
I. Wąsowska 1
 
 
 
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
 
Publication date: 2005-06-26
 
 
Corresponding author
M. Czauderna   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
 
J. Anim. Feed Sci. 2005;14(Suppl. 1):563-566
DOI: https://doi.org/10.22358/jafs/70737/2005
 
 
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Citations (3)
 
KEYWORDS
fatty acids
conjugated dienes
gas-liquid
silver ion chromatography
 
ABSTRACT
A method is proposed for routine determination of fatty acids (FAs), including conjugated fatty acids, in the liver of rats and sheep using gas-liquid chromatography (GLC) and Ag+-HPLC. The proposed procedure includes new methods for saponification carried out at room temperature and extraction of free FAs. The obtained free FAs are methylated in an acid-base catalyzed procedure. Due to simultaneous fractionation of methylated FAs using GLC and Ag+-HPLC, the accuracy and sensitivity of the procedure is improved (especially for conjugated linoleic acids).
 
CITATIONS (3):
1.
Effect of dietary conjugated linoleic acid and selenized yeast on the concentration of fatty acids and minerals in rats
Marian Czauderna, Jan Kowalczyk, Katarzyna Korniluk
Archives of Animal Nutrition
 
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2.
The analysis of lamb and beef fatty acids with a 30 m BPX70 column is comparable with a 120 m column
Edward H. Clayton, Benjamin W. B. Holman, Richard G. Meyer
 
CrossRef
 
 
3.
The analysis of lamb and beef fatty acids with a 30 m BPX70 column is comparable with a 120 m column
Edward H Clayton, Benjamin W.B. Holman, Richard G Meyer
Journal of Food Composition and Analysis
 
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