0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

Gene expression in bovine mammary somatic cells isolated from milk

A. A. Hayashi 1,  
S. A. McCoard 1  ,  
N. C. Roy 1,  
 
1
Nutrition and Behaviour Group, AgResearch Limited
2
Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
J. Anim. Feed Sci. 2004;13(Suppl. 1):401–404
Publish date: 2004-08-30
KEYWORDS
ABSTRACT
Traditionally, in order to examine the expression of genes involved in mammary gland metabolism, RNA was prepared from mammary biopsies or from tissue samples taken at slaughter. The objective of this study was to validate the use of milk somatic cells as a model to study gene expression in the lactating bovine mammary gland. The expression pattern of milk protein and epithelial cell marker genes was similar between somatic cells and mammary tissue indicating that cells exfoliated into milk provide a viable non-invasive tissue source for gene profiling in the bovine mammary gland.
CORRESPONDING AUTHOR
S. A. McCoard   
Nutrition and Behaviour Group, AgResearch Limited
 
CITATIONS (6):
1. Evaluation of a replacement method for mammary gland biopsies by comparing gene expression in udder tissue and mammary epithelial cells isolated from milk
K. Krappmann, R. Weikard, C. Kühn
Research in Veterinary Science
2. Use of milk epithelial cells to study regulation of cell activity and apoptosis during once-daily milking in goats
H. Ben Chedly, P. Lacasse, P.-G. Marnet, M. Komara, S. Marion, M. Boutinaud
animal
3. Expression Profiling of Glucose Transporter 1 (GLUT1) and Apoptotic Genes (BAX and BCL2) in Milk Enriched Mammary Epithelial Cells (MEC) in Riverine Buffalo during Lactation
Poonam Yadav, Desh Deepak Singh, Manishi Mukesh, R. S. Kataria, Anita Yadav, A. K. Mohanty, B. P. Mishra
Animal Biotechnology
4. Unilateral once daily milking locally induces differential gene expression in both mammary tissue and milk epithelial cells revealing mammary remodeling
Marion Boutinaud, Laurent Galio, Vanessa Lollivier, Laurence Finot, Sandra Wiart, Diane Esquerré, Eve Devinoy
Physiological Genomics
5. Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Claudia Bevilacqua, Samira Makhzami, Jean-Christophe Helbling, Pierre Defrenaix, Patrice Martin
BMC Cell Biology
6. Milking and Feed Restriction Regulate Transcripts of Mammary Epithelial Cells Purified from Milk
M. Boutinaud, M.H. Ben Chedly, E. Delamaire, J. Guinard-Flament
Journal of Dairy Science
ISSN:1230-1388