We investigated mRNA expression of the glucose transporter, SGLT1, and the long-chain fatty acids translocase, CD36, in the gastrointestinal tract of cattle and goats before and after weaning. SGLT1 and CD36 expression in the jejunum was highest in the gastrointestinal tract before weaning, but it decreased in the small intestine after weaning in both species. From these results, we conclude that the jejunum is likely to be the major absorption site for glucose and long-chain fatty acids before weaning, and the expression of both types of transporters decrease in cattle and goats after weaning.