ORIGINAL PAPER
Changes of β-carotene and retinol levels and BCO1 gene and protein expressions in yak tissues
at different nutritional seasons
1
Qinghai University, Agriculture and Animal Husbandry College, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
2
Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
3
Qinghai University, Institute of Animal and Veterinary Science, 1 Weier road, Biotechnology park,
Chengbei districtrict, Xining city, Qinghai province, 810016, China
Publication date: 2019-06-19
Corresponding author
S.-L. Wang
Qinghai University, Agriculture and Animal Husbandry College, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
Qinghai University, Agriculture and Animal Husbandry College, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
J. Anim. Feed Sci. 2019;28(2):120-127
KEYWORDS
TOPICS
ABSTRACT
In order to explore relationship between the yellow fat and
β-carotene metabolism in yaks, contents of β-carotene and retinol in tissues,
serum and digestive tract contents were analysed at periods of rich and deficient
nutrition. Expressions of β-carotene 15, 15’-monooxygenase 1 (BCO1) gene
and protein were also studied using real-time PCR and western blot. It was
shown that contents of β-carotene in serum, liver, pancreas, duodenum, jejunum
and contents of rumen and ileum were higher in the period of rich nutrition than
in the period of deficient nutrition (P < 0.05). Content of retinol in liver was
much higher in the period of rich nutrition than in the period of deficient nutrition
(P < 0.05). Expression levels of BCO1 mRNA in kidney, jejunum, ileum,
duodenum, muscle and rumen in the period of rich nutrition were significantly
lower than in the period of deficient nutrition (P < 0.05), besides pancreas.
Expressions of BCO1 protein in duodenum, jejunum, kidney, muscle, and rumen
were lower in the period of rich nutrition than in the period of deficient nutrition
(P < 0.05).
REFERENCES (32)
1.
Amengual J., Widjaja-Adhi M.A.K., Rodriguez-Santiago S., Hessel S., Golczak M., Palczewski K., von Lintig J., 2013. Two carotenoid oxygenases contribute to mammalian provitamin A metabolism. J Biol Chem. 288, 34081–34096, https://doi.org/10.1074/jbc.M1....
2.
Bachmann H., Desbarats A., Pattison P., Sedgewick M., Riss G., Wyss A., Cardinault N., Duszka C., Goralczyk R., Grolier P., 2002. Feedback regulation of β, β-carotene 15, 15’-monooxygenase by retinoic acid in rats and chickens. J. Nutr. 132, 3616–3622, https://doi.org/10.1093/jn/132....
3.
Burri B.J., Clifford A.J., 2004. Carotenoid and retinoid metabolism: insights from isotope studies. Arch. Biochem. Biophys. 430, 110–119. https://doi.org/10.1016/j.abb.....
4.
Dag I.V., Inger A.B., 2010. A nonsense mutation in the beta-carotene oxygenase 2 (BCO2) gene is tightly associated with accumulation of carotenoids in adipose tissue in sheep (Ovis aries). BMC Genet. 11, 10. https://doi.org/10.1186/1471-2....
5.
Dolt K.S., Mishra M.K., Karar J., Baig M.A., Ahmed Z., Pasha M.A.Q., 2007. cDNA cloning, gene organization and variant specific expression of HIF-1α in high altitude yak (Bos grunniens). Gene 386, 73–80. https://doi.org/10.1016/j.gene....
6.
Glover J., 1960. The conversion of beta-carotene into vitamin A. Vitam. Horm. 18, 371-186. https://doi.org/10.1016/S0083-....
7.
Gou R.F., Wang R., Chai S.T., Hao L.Z., Wang S. L., 2016. Comparison of β-carotene and vitamin A contents in yak tissues in nutrition rich and deficiency periods (in Chinese). Acta Nutr. Sinica 38, 191–194, https://doi.org/10.13325/j.cnk....
8.
Hessel S., Eichinger A., Isken A. et al., 2007. CMO1 deficiency abolishes vitamin A production from β-carotene and alters lipid metabolism in mice. J. Biol. Chem. 282, 33553–33561, https://doi.org/10.1074/jbc.M7....
9.
Hickenbottom S.J., Follett J.R., Lin Y., Dueker S.R., Burri B.J., Neidlinger T R., Clifford A.J., 2002. Variability in conversion of β-carotene to vitamin A in men as measured by using a double-tracer study design. Amer. J. Clin. Nutr. 75, 900–907. https://doi.org/10.1093/ajcn/7....
10.
Kam R.K.T., Deng Y., Chen Y., Zhao H., 2012. Retinoic acid synthesis and functions in early embryonic development. Cell Biosci. 2, 11, https://doi.org/10.1186/2045-3....
11.
Kiefer C., Hessel S., Lampert J.M., et al. 2001. Identification and characterization of a mammalian enzyme catalyzing the asymmetric oxidative cleavage of provitamin A. J. Biol. Chem. 276, 14110-14116. https://doi.org/10.1074/jbc.M0....
12.
Lemke S.L., Dueker S.R., Follett J.R., Lin Y., Carkeet C., Buchholz B.A., Vogel J.S., Clifford A.J., 2003. Absorption and retinol equivalence of β-carotene in humans is influenced by dietary vitamin A intake. J. Lipid Res., 44, 1591–1600, https://doi.org/10.1194/jlr.M3....
13.
Lindqvist A., Andersson S., 2002. Biochemical properties of purified recombinant human β-carotene 15,15′-monooxygenase. J. Biol. Chem. 277, 23942–23948, https://doi.org/10.1074/jbc.M2....
14.
Lindqvist A., Sharvill J., Sharvill D.E., Andersson S., 2007. Loss of function mutation in carotenoid 15,15’-monooxygenase identified in a patient with hypercarotenemia and hypovitaminosis A. J. Nutr. 137, 2346–2350, https://doi.org/10.1093/jn/137....
15.
Morales A., González A., Varela-Echavarría A., Shimada A., Mora O., 2007. Differences in expression and activity of β,β′-carotene15,15′-oxygenase in liver and duodenum of cattle with yellow or white fat. J. Anim. Physiol. Anim. Nutr. 91, 341–346, https://doi.org/10.1111/j.1439....
16.
Moren M , Næss T , Hamre K., 2002. Conversion of β-carotene, canthaxanthin and astaxanthin to vitamin A in Atlantic halibut (Hippoglossus hippoglossus L.) juveniles. Fish Physiol. Biochem. 27, 71–80, https://doi.org/10.1023/B:FISH....
17.
Redmond T.M., Gentleman S., Duncan T., Yu S., Wiggert B., Gantt E., Cunningham Jr. F.X., 2001. Identification, expression, and substrate specificity of a mammalian β-carotene 15,15’-dioxygenase. J. Biol. Chem. 276, 6560–6565, https://doi.org/10.1074/jbc.M0....
18.
Schweigert F.J., Buchholz I., Bonitz K., 1998. Effect of age on the levels of retinol and retinyl ester in blood plasma, liver and kidney of dogs. Int. J. Vitam. Nutr. Res. 68, 237-241. https://doi.org/10.1016/S0308-....
19.
Shao B., Long R., Ding Y., Wang J., Ding L., Wang H., 2010. Morphological adaptations of yak (Bos grunniens) tongue to the foraging environment of the Qinghai-Tibetan Plateau. J. Anim. Sci. 88, 2594–2603, https://doi.org/10.2527/jas.20....
20.
Van Helden Y.G.J., Heil S.G., Van Schooten F.J. et al., 2010. Knockout of the Bcmo1 gene results in an inflammatory response in female lung, which is suppressed by dietary beta-carotene. Cell. Mol. Life Sci. 67, 2039–2056, https://doi.org/10.1007/s00018....
21.
Van Soest P.J., 1994. Nutritional Ecology of the Ruminant. Cornell University Press. Ithaca (USA).
22.
Von Lintig J., Wyss A., 2001. Molecular analysis of vitamin A formation: cloning and characterization of β-carotene 15,15’-dioxygenases. Arch. Biochem. Biophys. 385, 47–52, https://doi.org/10.1006/abbi.2....
23.
Wang H., Long R., Liang J.B., Guo X., Ding L., Shang Z., 2011. Comparison of nitrogen metabolism in Yak (Bos grunniens) and indigenous cattle (Bos taurus) on the Qinghai-Tibetan Plateau. Asian-Australas. J. Anim. Sci. 24, 766–773, https://doi.org/10.5713/ajas.2....
24.
Wang L.-H., Huang S.-H., 2002. Determination of vitamins A, D, E, and K in human and bovine serum, and β-carotene and vitamin A palmitate in cosmetic and pharmaceutical products, by isocratic HPLC. Chromatographia 55, 289–296, https://doi.org/10.1007/BF0249....
25.
Wang X.D., 1994. Review: absorption and metabolism of betacarotene. J. Amer. College Nutr. 13, 314–325, https://doi.org/10.1080/073157....
26.
Wang Z., Yin S., Zhao X., Russell R.M., Tang G., 2004. β-carotenevitamin A equivalence in Chinese adults assessed by an isotope dilution technique. Brit. J. Nutr. 91, 121–131, https://doi.org/10.1079/BJN200....
27.
Wiener G., Han J.L., Long R.J., 2003. The yak (2nd Edition). Bangkok, Regional Office for Asia and the Pacific Food and Agriculture Organization of the United Nations. Bangkok (Thailand).
28.
Wyss A., Wirtz G., Woggon W.-D., Brugger R., Wyss M., Friedlein A., Bachmann H., Hunziker W., 2000. Cloning and expression of β, β-carotene 15,15’-dioxygenase. Biochem. Biophys. Res. Commun. 271, 334–336, https://doi.org/10.1006/bbrc.2....
29.
Wyss A., Wirtz G.M., Woggon W.-D., Brugger R., Wyss M., Friedlein A., Riss G., Bachmann H., Hunziker W., 2001. Expression pattern and localization of β,β-carotene 15,15′-dioxygenase in different tissues. Biochem. J. 354, 521–529, https://doi.org/10.1042/bj3540....
30.
Xue B., Zhao X.Q., Zhang Y.S., 2005. Seasonal changes in weight and body composition of yak grazing on alpine-meadow grassland in the Qinghai-Tibetan plateau of China. J. Anim. Sci. 83, 1908–1913, https://doi.org/10.2527/2005.8....
31.
Yang A., Larsen T.W., Tume R.K., 1992. Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle. Austr. J. Agri. Res. 43, 1809–1817, https://doi.org/10.1071/AR9921....
32.
Yao B.-X., Zeng Q.-P., Xiao F.-Y., Yu L.-H., Fu J., Weng W., 2008. Analysis of the content of vitamin A in several animal livers. App. Chem. Industr. 37, 953–954.
CITATIONS (5):
1.
Retinol deficiency in animals: Etiopathogenesis and consequences
P. Skliarov, S. Fedorenko, S. Naumenko, O. Onischenko, K. Holda
Regulatory Mechanisms in Biosystems
P. Skliarov, S. Fedorenko, S. Naumenko, O. Onischenko, K. Holda
Regulatory Mechanisms in Biosystems
2.
Retinol deficiency in animals: Etiopathogenesis and consequences
P. Skliarov, S. Fedorenko, S. Naumenko, O. Onischenko, K. Holda
Regulatory Mechanisms in Biosystems
P. Skliarov, S. Fedorenko, S. Naumenko, O. Onischenko, K. Holda
Regulatory Mechanisms in Biosystems
3.
Jejunal barrier dysfunction of Datong Yak with high proportion concentrate dietary during the cold season
Jianlei Jia, Chunnian Liang, Xiaoyun Wu, Lin Xiong, Pengjia Bao, Qian Chen, Ping Yan
Jianlei Jia, Chunnian Liang, Xiaoyun Wu, Lin Xiong, Pengjia Bao, Qian Chen, Ping Yan
4.
Effect of high proportion concentrate dietary on Yak jejunal structure, physiological function and protein composition during cold season
Jianlei Jia, Chunnian Liang, Xiaoyun Wu, Lin Xiong, Pengjia Bao, Qian Chen, Ping Yan
Scientific Reports
Jianlei Jia, Chunnian Liang, Xiaoyun Wu, Lin Xiong, Pengjia Bao, Qian Chen, Ping Yan
Scientific Reports
5.
High proportion concentrate dietary brings to Ashdan Yak jejunal barrier and microbial dysfunction during cold season
Jianlei Jia, Chunnian Liang, Xiaoyun Wu, Lin Xiong, Pengjia Bao, Qian Chen, Ping Yan
Research in Veterinary Science
Jianlei Jia, Chunnian Liang, Xiaoyun Wu, Lin Xiong, Pengjia Bao, Qian Chen, Ping Yan
Research in Veterinary Science
Share
RELATED ARTICLE
| ISSN: | 1230-1388 |
Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
