0.917
IF5
1.024
IF
Q2
JCR
0.90
CiteScore
0.385
SJR
Q2
SJR
20
MNiSW
142.18
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Can xylene and chia (Salvia hispanica L.) seed extract directly affect basic bovine ovarian cell functions?

A. Tarko 1  ,  
A. Stochmalova 1  ,  
S. Hrabovszka 1  ,  
A. Vachanova 1  ,  
A. H. Harrath 2  ,  
R. Grossman 3  ,  
A. Sirotkin 1  
 
1
Constantine the Philosopher University in Nitra, 949 74 Nitra, Slovakia
2
King Saud University, Riyadh 12372, Saudi Arabia
3
Friedrich Loeffner Institute, Mariensee, 31535 Neustadt am Rübenberge, Germany
J. Anim. Feed Sci. 2017;26(2):109–115
Publish date: 2017-06-14
KEYWORDS:
TOPICS:
ABSTRACT:
It was supposed, that xylene as environmental contaminant and chia (Salvia hispanica L.) as a medicinal plant may affect ovarian cell functions, and that chia seed extract could modify the potentially adverse effect of xylene. So, the aim of our investigation was to study the effect of xylene, extract of chia seed and their combination on bovine ovarian cell functions (proliferation, apoptosis and secretory activity) in vitro. Proliferation, apoptosis and the release of hormones (insulin-like growth factor 1 (IGF-1), progesterone and testosterone) were analysed with the use of either immunocytochemistry or EIA/RIA. It was observed, that xylene when given alone stimulated proliferation and did not influence the apoptosis. Furthermore, xylene inhibited release of progesterone and testosterone but did not change the release of IGF-1. Chia seed extract inhibited proliferation, apoptosis and the release of IGF-1, progesterone and testosterone. Moreover, chia seed extract suppressed the stimulatory effect of xylene on proliferation, and added together stimulated apoptosis. The simultaneous addition of chia seed extract and xylene stimulated IGF-1 release and inhibited the release of progesterone, but not testosterone. The obtained results prove a direct effect of both xylene and chia seed extract on bovine ovarian cell proliferation, apoptosis and secretory activity. Moreover, it is the first study in which chia seed extract was able to suppress xylene effect on ovarian cell proliferation. However, it prevented only from one xylene effect among five analysed suggesting that chia seed extract could not be potentially useful for natural prevention of all negative effects of xylene on reproduction.
CORRESPONDING AUTHOR:
A. Tarko   
Constantine the Philosopher University in Nitra, 949 74 Nitra, Slovakia
 
REFERENCES (20):
1. Arp E.W Jr., Wolf P.H., Checkoway H., 1983. Lymphocytic leukemia and exposures to benzene and other solvents in the rubber industry. J. Occup. Med. 25, 598–602.
2. Connolly K.M., Bogdanffy M.S., 1993. Evaluation of proliferating cell nuclear antigen (PCNA) as an endogenous marker of cell proliferation in rat liver: a dual-stain comparison with 5-brom-2’-deoxyuridine. J. Histochem. Cytochem. 41, 1–6, https://doi.org/10.1177/41.1.7678022.
3. Huang S.-T., Chen A.P.-C., 2008. Traditional Chinese medicine and infertility. Curr. Opin. Obstet. Gyn. 20, 211–215, https://doi.org/10.1097/gco.0b013e3282f88e22.
4. Ixtaina V.Y., Martínez M.L., Spotorno V., Mateo C.M., Maestri D.M., Diehl B.W.K., Nolasco S.M., Tomás M.C., 2011. Characterization of chia seed oils obtained by pressing and solvent extraction. J. Food Comp. Anal. 24, 166–174, https://doi.org/10.1016/j.jfca.2010.08.006.
5. Kim I.Y., Kim B.-C., Seong D.H., Lee D.K., Seo J.-M., Hong Y.J., Kim H.-T., Morton R.A., Kim S.-J., 2002. Raloxifene, a mixed estrogen agonist/antagonist, induces apoptosis in androgenindependent human prostate cancer cell lines. Cancer Res. 62, 5365–5369.
6. Liang Z.-H., Yin D.-Z., 2010. Preventive treatment of traditional Chinese medicine as antistress and antiaging strategy. Rrejuv. Res. 13, 248–252, https://doi.org/10.1089/rej.2009.0867.
7. Makarevich A., Sirotkin A., 1999. Development of a sensitive radioimmunoassay for IGF–I determination in samples from blood plasma and cell–conditioned medium. Vet. Med. 44, 71–78.
8. Münster E., 1989. Development of enzymatic-immunologic measuring techniques on micro-titration plates for determining testosterone and progesterone in the blood plasma (in German). PhD Thesis. Institute for Animal Production and Breeding, University of Hohemheim, Stuttgart (Germany).
9. Osborn M., Isenberg S., 1994. Immunocytochemistry of frozen and of paraffin tissue sections. In: J.E. Celis (Editor). Cell Biology: A Laboratory Handbook. Academic Press. San Diego, CA (USA), pp. 361–367.
10. Prakash B.S., Meyer H.H.D., Schallenberger E., van De Wiel D.F.M., 1987. Development of a sensitive enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique. J. Steroid Biochem. 28, 623–627, https://doi.org/10.1016/0022-4731(87)90389-X.
11. Reyes-Caudillo E., Tecante A., Valdivia-López M.A., 2008. Dietary fibre content and antioxidant activity of phenolic compounds present in Mexican chia (Salvia hispanica L.) seeds. Food Chem. 107, 656–663, https://doi.org/10.1016/j.foodchem.2007.08.062.
12. Sirotkin A.V., 2010. Effect of two types of stress (heat shock/high temperature and malnutrition/serum deprivation) on porcine ovarian cell functions and their response to hormones. J. Exp. Biol. 213, 2125–2130, https://doi.org/10.1242/jeb.040626.
13. Sirotkin A.V., 2011. Regulators of Ovarian Functions. Nova Science Publishers, Inc. Hauppauge, NY (USA).
14. Sirotkin A.V., Harrath A.H., 2014. Phytoestrogens and their effects. Eur. J. Pharmacol. 741, 230-236, https://doi.org/10.1016/j.ejphar.2014.07.057.
15. Sirotkin A.V., Kádasi A., Baláži A., Baková Z., Harrath H.A., Makarevich A.V., Kolesárová A., Chrenek P., Kotwica J., Tóth T., 2012. Influence of petrochemical industry environmental contaminants on animal ovarian cells. J. Microbio. Biotechnol. Food Sci. 2, 517–525.
16. Sirotkin A.V., Kadasi A., Stochmalova A., Alexa R., Balazi A., Harrath A.H., 2015. Food, drink and medical plants and their molecules can affect female hormones and reproductive functions. Endocr. Abstr. 38, P370, https://doi.org/10.1530/endoabs.38.P370.
17. Steiner M.S., Pound C.R., 2003. Phase IIA clinical trial to test the efficacy and safety of Toremifene in men with high-grade prostatic intraepithelial neoplasia. Clin. Prostate Cancer 2, 24–31, https://doi.org/10.3816/CGC.2003.n.009.
18. Štochmaľová A., Kádasi A., Alexa R., Sirotkin A.V., 2013. The effect of quercetin and FSH o proliferation and apoptosis of porcine ovarian granulosa cells. The 4th International Scientific Conference. Applied Natural Sciences. 1st Edition. 1 CD-ROM [262 s.]. ISBN 978-80-8105-502-7. Applied natural sciences. Trnava: Univerzita sv. Cyrila a Metoda, 2013, CD-ROM, s. 230-235, http://www.slpk.sk/form/epc/prehlady/2014/FBP2014.pdf.
19. Taskinen H., Kyyrönen P., Hemminki K., Hoikkala M., Lajunen K., Lindbohm M.-L., 1994. Laboratory work and pregnancy outcome. J. Occup. Med. 36, 311–319, https://doi.org/10.1097/00043764-199403000-00008.
20. Ungváry G., Varga B., Horváth E., Tátrai E., Folly G., 1981. Study on the role of maternal sex steroid production and metabolism in the embryotoxicity of para-xylene. Toxicology 19, 263–268, https://doi.org/10.1016/0300-483X(81)90136-0.
ISSN:1230-1388