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An improved method for derivatization of fatty acids for liquid chromatography
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna Poland
 
 
Publication date: 2001-06-28
 
 
J. Anim. Feed Sci. 2001;10(Suppl. 2):369-375
 
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ABSTRACT
Derivatization of fatty acids is usually carried out with 2,4'-dibromoacetophenone in the presence of excess of basic species at 50 °C for 2 h. Under such conditions, some unsaturated fatty acids can be oxidized or isomerized. To prevent oxidation or isomerization, polyunsaturated fatty acids, particularly conjugated dienes, should be derivatized at ~ -26°C for 6.5 h. The proposed new procedure assures a decrease of degradation or isomerization of unsaturated fatty acids. This procedure provides a simple method for preparation and derivatization of fatty acids in milk, meat, fat and intestinal digesta followed by HPLC analysis with UV detection.
 
CITATIONS (1):
1.
Analysis of fatty acids by column liquid chromatography
Shu-Hui Chen, Yen-Jun Chuang
Analytica Chimica Acta
 
ISSN:1230-1388
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