ORIGINAL PAPER
Addition of coconut oil to the diet based on maize dried
distilled grains with solubles (DDGS) alters miR-122a expression
in the pig liver
1
National Research Institute of Animal Production, Department of Animal Genetics and Breeding, Krakowska 1, 32-083 Balice, Poland
2
National Research Institute of Animal Production, Laboratory of Genomics,
Krakowska 1, 32-083 Balice, Poland
Publication date: 2017-12-10
Corresponding author
M. Oczkowicz
National Research Institute of Animal Production, Department of Animal Genetics and Breeding, Krakowska 1, 32-083 Balice, Poland
National Research Institute of Animal Production, Department of Animal Genetics and Breeding, Krakowska 1, 32-083 Balice, Poland
J. Anim. Feed Sci. 2017;26(4):326-332
KEYWORDS
TOPICS
ABSTRACT
The aim of the study was to analyse the expression of several
microRNAs (miRNA) in the liver of pigs fed different diets. Twenty-four fatteners
(12 gilts and 12 barrows) originating from (Polish Landrace × White Large Polish)
sows mated with (Duroc × Pietrain) boars were divided into 4 dietary groups,
with 6 pigs in each group (3 gilts and 3 barrows). The fattening experiment
lasted from about 60 kg to 118 kg of body weight. The animals were fed diets
that differ with the presence of maize dried distilled grains with solubles (DDGS;
groups II, III, IV – 20%) and the type of used fat (rapeseed oil – groups I and
II, beef tallow – group III, coconut oil – group IV). A qPCR analysis to assess
miR-148a-3p, miR-122a, miR-26a, miR-103, let-7a, miR-92a, miR-335 and
miR-16a expressions was performed. In the GeNorm software analysis it was
shown that the most stably expressed miRNAs were miR-26a, miR-16a and
miR-148a-3p (M values: 0.51–0.52). Only miR-122a expression was different
(P < 0.03). The miR-122a level was statistically lower in the liver of pigs from
group IV (DGS+coconut oil). The results suggest that adding coconut oil to
feedstuff based on maize DDGS changes the expression of miR-122a, which is
an important regulator of many genes engaged in lipid metabolism.
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