ORIGINAL PAPER
5’-flanking variants of the equine α-lactalbumin (LALBA) gene – relationship with gene expression and mare’s milk composition
1
Poznań University of Life Sciences, Department of Horse Breeding, Department of Genetics and Animal Breeding, Wołyńska 33, 60-637 Poznań, Poland
2
Poznań University of Life Sciences, Department of Genetics and Animal Breeding, Wołyńska 33, 60-637 Poznań, Poland
3
Warsaw University of Life Sciences – SGGW, Department of Animal Science, Cattle Breeding Division
Ciszewskiego 8, 02-786 Warsaw, Poland
4
Poznań University of Life Sciences, Department of Animal Breeding and Product Quality Assessment
Złotniki, Słoneczna 1, 62-002 Suchy Las, Poland
Publication date: 2018-11-28
Corresponding author
J. Cieślak
Poznań University of Life Sciences, Department of Horse Breeding, Department of Genetics and Animal Breeding, Wołyńska 33, 60-637 Poznań, Poland
Poznań University of Life Sciences, Department of Horse Breeding, Department of Genetics and Animal Breeding, Wołyńska 33, 60-637 Poznań, Poland
J. Anim. Feed Sci. 2018;27(4):317-326
KEYWORDS
TOPICS
ABSTRACT
α-Lactalbumin (α-LA) is one of the most abundant milk whey
proteins among different mammalian species including domestic horse. The aim
of this study was to screen for polymorphism in the 5’-flanking region of the
equine α-LA (LALBA) gene and to assess the potential relationship of particular
genotypes with LALBA gene expression variability (measured at the mRNA and
protein levels) and with basic milk composition traits. Initial screening for LALBA
gene 5’-flanking variants was conducted using direct sequencing of DNA derived
from 96 horses representing 12 breeds (Polish Primitive Horse, Polish Coldblood
Horse, Polish Warmblood Horse, Silesian, Hucul, Fiording, Haflinger, Shetland
Pony, Welsh Pony, Arabian, Thoroughbred and Percheron). Association analysis
of detected polymorphisms, gene expression and milk composition traits was
carried out for 74 horses (Polish Primitive Horse, Polish Coldblood Horse and
Polish Warmblood Horse breeds). Altogether 4 single nucleotide polymorphisms
(SNPs) (c.−165G>C, c.−222A>G, c.−357C>A and c.−928C>T) were found in
the LALBA gene 5’-flanking region (NC_009149.3 GenBank sequence, gene
coordinates on ECA6: 67372475–67375877). Although bioinformatic prediction
suggested that 3 of them may alter the consensus sequences for transcription
factors, no significant associations between genotypes and LALBA gene
expression were recorded. However, a significant relationship (P < 0.05) was
noticed for c.−928C>T SNP genotypes and basic milk composition (fat and
protein contents) of Polish Primitive Horse mares. Additionally, in our study the
significant impact of horse breed and lactation period on LALBA gene expression
and basic milk composition traits was revealed.
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